농산물 중 Staphylococcus aureus의 분리를 위한 선택배지 평가

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 27권 / 2호
· 저자명 : 김세리, 이서현, 서민경, 김원일, 박경훈, 윤혜정, 윤요한, 유순영, 류경열, 윤종철, 김병석

목차

ABSTRACT
서 론
재료 및 방법
균주와 배지
선택배지의 민감도와 특이도 분석
선택배지의 회복능 조사
농산물에서 회수율 조사
통계처리
결과 및 고찰
민감도와 특이도분석
선택배지의 회복능 및 농산물에서 회수능 조사
요 약
감사의 글
참고문헌

영어초록

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird- Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat (60oC for 90s), and cold (−20oC for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

참고자료

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