Medium Supplementation with Rare Earth Elements Reduces Hyperhydricity during Adventitious Shoot Multiplication In Vitro of Carnation

서지정보

· 발행기관 : 한국화훼학회
· 수록지 정보 : 화훼연구 / 26권 / 1호
· 저자명 : Bo Ling Liu, Hao Wei, Ji Eun Park, Byoung Ryong Jeong

목차

Abstract
Introduction
Materials and Methods
Medium preparation and culture conditions
Malondialdehyde (MDA) concentrations
Enzyme extraction and assay
SOD activity
CAT activity
GPX activity
APX activity
Superoxide radical (O2-) staining
Results
Effect of REEs on the hyperhydricity ratio and induction rates of carnation
Effect of REEs on soluble protein concentration
Effect of REE on MDA concentrations
Effect of REE on activities of antioxidative enzymes
Discussion
REE supplementation results in reduced hyperhydricity rate during the tissue culture
Regulation of antioxidant enzymes by REEs in response to the oxidative stress
References

영어초록

Dianthus caryophyllus (carnation) is a globally important ornamental plant. Tissue culture techniques have been used for the commercial production of carnation; however, the micropropagation of carnation has been impeded due to the occurrence of hyperhydricity during the shoot multiplication process or micropropagation stage 2. In the present study, the effects of different concentrations of rare earth elements on the reduction of hyperhydricity in micropropagated carnation were investigated. Nodal explants of D. caryophyllus ‘13827’ were cultured on Murashige and Skoog medium containing 1.0 mg·L-1 6-benzyladenine and 0.5 mg·L-1 indole-3-acetic acid with 3.0% (w/v) sucrose and 0.8% (w/v) agar (control medium). The medium was supplemented with lanthanum nitrate, La(NO3)3, cerium nitrate, Ce(NO3)3, or neodymium chloride, NdCl3 at a concentration of 0.05, 0.1, or 0.15 mM. Hyperhydricity was observed in 68.9% of the cultures produced on the control medium. The lowest percentage (42.2%) of hyperhydricity was observed in plants propagated on the medium supplemented with 0.05 mM Ce(NO3)3. Furthermore, the soluble protein concentration was higher in both the non-hyperhydric and hyperhydric plants produced on the medium supplemented with 0.05 mM Ce(NO3)3, whereas the activity of catalase, guaiacol peroxidase, and ascorbate peroxidase was lower in comparison with the media lacking Ce3+. The results of this study suggest that supplementation of the culture medium with 0.05 mM Ce(NO3)3 alleviates oxidative stress and reduces hyperhydricity in carnation during the adventitious shoot multiplication stage.

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