Protective effects of recombinant Brucella abortus protein Fe/Mn superoxide dismutase against a virulent strain B. abortus 544 infection in BALB/c mice

서지정보

· 발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
· 수록지 정보 : 예방수의학회지 / 46권 / 4호
· 저자명 : Tran Xuan Ngoc Huy, Trang Thi Nguyen, Alisha Wehdnesday Bernardo Reyes, HeeJin Kim, WonGi Min, Hu Jang Lee, John Hwa Lee, Suk Kim

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and growth condition
Recombinant protein expression and purification
Mice immunization and bacterial challenge
Quantitative measurement of CD4+ and CD8+ T cells
Bacterial clearance efficiency
Statistical analysis
RESULTS
Protein purification and immunoreactivity of recombinantprotein
rFe/Mn SOD induced the peripheral blood CD4+ andCD8+ T cell subsets proliferation
rFe/Mn SOD induced the protective effect against B.abortus in mice
DISCUSSION
ACKNOWLEDGEMENT
CONFLICT OF INTEREST STATEMENT
REFERENCES

영어초록

Subunit vaccines are being developed as a potential therapy for preventing microbial pathogen infection. In this study, the immunogenicity of recombinant Brucella (B.) abortus Fe/Mn superoxide dismutase (rFe/Mn SOD) protein as a subunit vaccine against B. abortus was investigated in BALB/c mice model. Brucella Fe/Mn SOD gene was cloned into a pcold-TF DNA vector. The bacterial recombinant protein was expressed using the Escherichia coli DH5α strain with a size of 82.50 kDa. The western blotting assay showed that rFe/Mn SOD reacted with Brucella-positive serum, indicating the potential immunoreactivity of this recombinant protein. After the second and third vaccinations, the peripheral CD4+ T cell population was increased significantly in the rFe/Mn SOD-immunized mice group compared to the PBS control group. Moreover, immunization of this recombinant protein increased the CD4+ T cell population from the first vaccination to the third vaccination. Meanwhile, the CD8+ T cells were slightly enhanced after the second vaccination compared to the first vaccination and compared to control groups. Fourteen days after the bacterial infection, the splenomegaly and the number of bacteria in the spleen were evaluated. The result showed that both rFe/Mn SOD and positive control RB51 decreased the bacterial replication in the spleen and the splenomegaly compared to control groups. Altogether, these results suggested that rFe/Mn SOD could induce host immunity against B. abortus infection.

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