Molecular Sexing and Species Identification of the Processed Meat and Sausages of Horse, Cattle and Pig

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 31권 / 1호
· 저자명 : Yoo-Kyung Kim, Yong-Jun Kang, Geun-Ho Kang, Pil-Nam Seong, Jin-Hyoung Kim, Beom-Young Park, Sang-Rae Cho, Dong Kee Jeong, Hong-Shik Oh, In-Cheol Cho, Sang-Hyun Han

목차

INTRODUCTION
MATERIALS AND METHODS
1. Samples and DNA Extraction
2. Molecular Sexing
3. DNA Sequencing
4. Species Identification
RESULTS AND DISCUSSION
REFERENCES

영어초록

We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock.

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