宿主昆虫을 利用한 담배거세미나방核多角體病바이러스의 大量生產

서지정보

· 발행기관 : 한국응용곤충학회
· 수록지 정보 : 한국응용곤충학회지 / 28권 / 2호
· 저자명 : 任大準, 崔鐀文, 李文弘, 陳炳來, 姜錫權

초록

담배거세미나방핵다각체병바이러스대량생산연구에서, 5영충에 1.1×107다각체/ml로 접종 후 8일에 수확했을 때 한 마리당 6.7×109 다각체를생산할 수 있었으며, 이때 통과는 한 마리당 약 2g의 인공통과로 충분하였다. 합성유약 호르몬인 methoprene (Manta˚ledR)을 처리했을 때 종영유충기간이 1~2일 연장되었으며, 바이러스 생산에 있어서도 무처리에 비해 약 15% 증가되었다.

영어초록

Mass production of Spodoptera litura nuclear polyhedrosis virus (SINPV) was carried out on massively reared host insects. The yield of SINPV was maximal with 6.7×109 PIBs per larva on the 8th day post inoculation, when 5th instar larvae were inoculated with 1.1×107 PIBs per ml, and 2 g of artificial diet was sufficient for food consumption of a larva. The moribund larvae were more suitable for handling and mass production of virus than the completely dead larvae. The larvae, when treated with methoprene (Manta˚ledR), prolonged their larval period and consequently became bigger to result in higher yield(about 15%) of virus.

참고자료

· 없음