Adaptation of the Hypoosmotic Swelling Test to Evaluate Membrane Integrity of Boar Spermatozoa

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 31권 / 2호
· 저자명 : Hyun-Yong Jang, Hee-Tae Cheong, Hwan-Sub Heang, Jong-Taek Kim, Choon-Keun Park, Hak-Kyu Lee, Boo-Keun Yang

목차

ABSTRACT INTRIDUSTION MATERIALS AND METHODS Semen Preparation Hypoosmotic Swelling Test(HOST) Comparison of Hypoosmotic Solutions Comparison of Incubation Time Comparison on Osmolarity Comparison of semen Status Quantification of Lipid Peroxidation Statistical Analysis RESULTS DISCUSSION REFERENCES

영어초록

The objective of this study was to establish the optimal conditions for hypoosmotic swelling (HOS) test to assess the functional integrity of the membranes of boar fresh or frozen/thawed spermatozoa. When pooled semen sample was incubated for 30 min at 37℃ with different test solution of varied osmolarity, the highest percentage of HOS positive spermatozoa was observed in a 150 mOsmol fructose/Na-citrate solution (33.6%). Incubation time did not affect significantly the score of HOS positive spermatozoa observed in a 150 mOsmol fructose/Na-citrate solution at 37℃, but the osmolarity affected the score of HOS positive spermatozoa under the same condition above. Fresh semen was significantly better than frozen/thawed semen in semen parameters evaluated such as motility, viability, membrane integrity and lipid peroxidation (p<0.05). In the relationships of sperm parameters, motility vs viability, motility vs membrane integrity and viability vs membrane integrity were positively correlated (0.82～0.94) but lipid peroxidation vs other estimated factors was negatively correlated (－0.90～－0.98). Among the evaluation methods, motility vs viability, motility vs membrane integrity and lipid peroxidation vs other estimated factors were significantly correlated (p<0.05). These results of this study indicate that the optimal condition of HOST in boar spermatozoa is a 150 mOsmol fructose/Na-citrate solution for 30 min incubation at 37℃ and HOST can substitute the examination of motility, viability and lipid peroxidation.

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