Gel electrophoresis and Sequencing (겔 전기영동과 시퀀싱), 영문 보고서

소개

"Gel electrophoresis and Sequencing (겔 전기영동과 시퀀싱), 영문 보고서"에 대한 내용입니다.
대학교 생화학실험 A+ 받은 레포트입니다. 영어로 작성되었습니다.
전기영동과 시퀀싱에 관한 내용이 있습니다. 시퀀싱 데이터 분석도 포함된 자료입니다. (소프트웨어로 BLAST X 사용)

목차

Abstraction
1. Introduction
2. Method
3. Results
4. Discussion
5. Reference

본문내용

We performed cloning in the previous experiments. And this time, an experiment was conducted to check whether cloning was done properly. Therefore, in experiment 4, sequencing and gel electrophoresis was performed to confirm the colony. As prior work, we performed recombinant DNA Mini-prep and restriction enzyme digestion. Then, sequencing and gel electrophoresis were done with the product of digestion. As a result of checking the gel electrophoresis experiment, two bands were observed in most colonies that we picked. As a result of analyzing the nucleotide sequence of sequencing, it was confirmed that the T4 DNA ligase gene was inserted into the colony. Our results show that the cloning was successful.

참고자료

· Jeremy M. Berg et al. 2019. Biochemistry. 9th ed. W.H. Freeman and Company.
· Campbell et al. 2019. Campbell Biology. 10th ed. Pearson.
· Moo-Sang Kim. NCBI– BLAST. [Department of Aquatic Life Medicine, Pukyong National University]
· “DNA ligase”. Solgent. 2019-08-16. http://www.solgent.com/sub03010202/view/id/36.
· What is the difference between forward and reverse primers”. PEDIAA. 2019-07-09, https://pediaa.com/what-is-the-difference-between-forward-and-reverse-primers/
· “Basic Sequencing”. BIONICS. http://www.bionicsro.co.kr/contents/serviceBasicsequencing
· Mitchell K, et al. (2020, March 17). Benchmarking of computational error-correction methods for next-generation sequencing data. Genome Biology
· “ClustalW Clustal Omega 사용방법”, tistory, last modified May 26. 2021, accessed Oct
· 28. 2022, https://arumyworld.tistory.com/53