[자연과학](예비실험보고서) SDS PAGE

소개

일반생물학 실험에서 사용한 SDS PAGE에 대한 예비보고서입니다.

목차

1. Procedure
2. Reducing SDS-PAGE
3. Electrophoresis and Staining
4. Buffer System

본문내용

Procedure
The solution of proteins to be analyzed is first mixed with SDS, an anionic detergent which denatures secondary and non–disulfide–linked tertiary structures, and applies a negative charge to each protein in proportion to its mass. Without SDS, different proteins with similar molecular weights would migrate differently due to differences in folding, as differences in folding patterns would cause some proteins to better fit through the gel matrix than others. Adding SDS solves this problem, as it linearizes the proteins so that they may be separated strictly by length (primary structure, or number of amino acids). The SDS binds to the protein in a ratio of approximately 1.4 g SDS per 1.0 g protein (although binding ratios can vary from 1.1-2.2 g SDS/g protein), giving an approximately uniform mass:charge ratio for most proteins, so that the distance of migration through the gel can be assumed to be directly related to only the size of the protein. A tracking dye may be added to the protein solution to allow the experimentor to track the progress of the protein solution through the gel during the electrophoretic run.

참고자료

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