In situ cross-linkable hyaluronic acid hydrogels prevent post-operative adhesions in rabbit model

소개

동시 가교시스템을 도입한 히알루론산 하이드로젤을 이용하여 조직유착방지막을 제조하여 연구한 논문입니다. 2006년도에 나왔으며 결과가 획기적이고 여러가지 데이타가 깨끗하여 1달만에 통과된 논문으로 MIT에서 연구했으며, 조직유착 방지막 연구하시는 분이 보시면 많은 참고 및 견본자료가 될 듯합니다.

목차

In situ cross-linkable hyaluronic acid hydrogels prevent post-operative
abdominal adhesions in a rabbit model

1. Introduction

2. Materials and methods
2.1. Materials
2.2. Preparation of cross-linkable HA
2.3. Characterization of hyaluronic acid hydrogels (HAXs)
2.4. In vitro cell viability assay
2.5. In vivo application of HAX gel
2.6. Effects of HA degradation products on tissue-type plasminogen
activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) production
2.7. Degradation of HAX gels of different concentrations in hyaluronidase
2.8. Statistical analysis

3. Results
3.1.1. Characterization of HA derivatives and cross-linked hydrogels
3.1.2. Cross-linked hydrogels
3.2. Cell viability
3.3.1. Prevention of peritoneal adhesions
3.3.2. Prevention of peritoneal adhesions
3.4. Histology
3.5. Effects of HA degradation products on tPA and PAI-1 production
3.6. Effect of HAX gel concentration on degradation

4. Discussion
The HAX gels for preventing post-operative adhesions
The advantages of HAX gel as barrier systems

5. Conclusions

본문내용

Post-operative adhesions are common and serious complications following surgery, as they can entail severe abdominal and pelvic pain, infertility, and bowel obstruction.

The advantages of HAX gel as barrier systems

1. Easy to handle and use
The solutions of HA derivatives are applied as mildly viscous liquids and instantly form a flexible hydrogel upon mixing.

2. Gelation occurs in a reasonably short time
(3 s to 3 min depending on the volume of two components and mixing methods) without requiring additional cross-linking agents such as initiators or light sources. Furthermore, gelation time can be tuned by altering the concentration of the components (data not shown).

3. Durable physical barrier for days to weeks
(depending on the concentration of the gel components, see Fig. 6), which are eventually degraded by endogenous hyaluronidase.

4. Compatible with mesothelial cells
in both non-degrading condition (plain medium) and degrading condition (medium containing 10 units/ml hyaluronidase).

참고자료

· In situ cross-linkable hyaluronic acid hydrogels prevent post-operative
· abdominal adhesions in a rabbit model,Biomaterials 27 (2006) 4698–4705