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Giemsa staining report, A+ 생명과학실험 레포트

생명과학부 전공 실험인 giemsa staining 실험에 대한 레포트입니다. 높은 레포트 점수를 받은 자료입니다.
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최초등록일 2023.08.08 최종저작일 2023.04
생명과학실험 생명과학레포트 레포트 giemsastaining
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Giemsa staining report, A+ 생명과학실험 레포트
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  • 미리보기

    소개

    생명과학부 전공 실험인 giemsa staining 실험에 대한 레포트입니다.
    높은 레포트 점수를 받은 자료입니다.

    목차

    I.Introduction
    1.Methylene blue
    2.Eosin
    3.Giemsa staining
    4.Romanowsky staining

    II.Result

    III. Discussion
    1.Sample A, B, C에 대한 discussion
    2.Staining을 통해 확인할 수 있는 cell type 별 morphology
    3.Giemsa staining 응용 및 활용 분야와 상세한 예시 2개 이상
    4.Methanol 이외에 cell fix에 사용될 수 있는 방법 및 원리

    본문내용

    Methylene blue (Methylthioninium chloride)는 hydrogen sulfide가 있을 때에 dimethyl-phenylene diamine을 ferric chloride와 함께 oxidization해서 만들어지는 thiazine dye이다. Thiazine은 organic compound로 4개의 탄소, 하나의 nitrogen, 하나의 sulfur atom을 포함하는 링 구조를 가진다. Sulphur와 nitrogen이 central ring에 붙어있으며, chlorine은 negatively charged로 분리된 그룹에 있다. Methylene blue는 물에 녹으면 acidic해지며 dark blue를 띈다. medication으로서 methemoglobinemia를 치료하기 위하여 사용되는데, hemoglobin 안의 ferric iron을 ferrous iron으로 전환하거나 화학적으로 억제한다.1 Antidepressant, cyanide poisoning에 대한 치료, titration indicator 등의 다양한 목적으로도 이용된다.
    Staining dye로서 methylene blue는 cationic stain이다. 이는 양전하를 띄고 acid를 만나면 파란 색조를 띈다. 음전하를 띄는 nucleus와 DNA, RNA의 nucleic acids 등의 세포 안의 구성물에 붙어서 파란 색으로 잘 식별되게 한다. nucleic acids에 대한 결합력으로 인해, northern blotting에서 RNA sequences를 식별하는 과정에 이용되기도 한다. Cellular structure, cell mortality를 확인하는 것에 이용될 수 있다. Methylene blue가 cell이나 tissue에 들어가면, 건강한 세포는 색이 사라지는데, 세포 안의 enzyme이 methylene blue를 분해하여 색을 잃게 하기 때문이다.

    참고자료

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  • AI와 토픽 톺아보기

    • 1. Methylene blue
      Methylene blue is a widely used synthetic dye in various fields, including biology, medicine, and chemistry. It is a versatile compound with a range of applications, from staining biological samples to serving as an oxidation-reduction indicator. In the field of biology, methylene blue is commonly used as a stain for microscopic examination of cells and tissues, particularly in the study of cellular structures and processes. Its ability to selectively bind to certain cellular components, such as nucleic acids and mitochondria, makes it a valuable tool for visualizing and analyzing cellular morphology and function. Additionally, methylene blue has been explored for its potential therapeutic applications, including its use as a treatment for certain medical conditions, such as methemoglobinemia and cyanide poisoning. Overall, methylene blue's diverse applications and its importance in various scientific and medical fields make it a significant topic of study and research.
    • 2. Eosin
      Eosin is a widely used synthetic dye in histology and cytology, primarily employed as a counterstain in combination with other dyes, such as hematoxylin. Its primary function is to stain the cytoplasm and extracellular matrix of cells, providing contrast and enhancing the visualization of cellular structures. Eosin is particularly useful in the identification and differentiation of various cell types, as it can selectively bind to different cellular components, such as proteins and lipids. This property makes eosin an essential tool in the analysis of tissue samples, enabling pathologists and researchers to better understand the morphological and structural characteristics of cells and tissues. Additionally, eosin's ability to stain the extracellular matrix can provide valuable information about the organization and composition of the surrounding tissue environment. The versatility and widespread use of eosin in various fields of biology and medicine make it a crucial topic of study and research.
    • 3. Giemsa staining
      Giemsa staining is a widely used technique in hematology and parasitology for the identification and differentiation of various cell types, particularly blood cells and parasites. The Giemsa stain is a complex mixture of methylene blue, eosin, and azure dyes, which collectively stain different cellular components with varying intensities, creating a distinctive color pattern that allows for the identification of specific cell types and their characteristics. This staining method is particularly valuable in the diagnosis and study of blood-borne diseases, such as malaria, where the identification of parasites within red blood cells is crucial for accurate diagnosis and treatment. Additionally, Giemsa staining is commonly used in cytogenetic analysis, where it can help visualize chromosomal structures and patterns, aiding in the detection of chromosomal abnormalities. The versatility and specificity of Giemsa staining make it an indispensable tool in various fields of biology and medicine, and its continued study and refinement are essential for advancing our understanding of cellular and molecular processes.
    • 4. Romanowsky staining
      Romanowsky staining is a widely used technique in hematology and cytology for the differential staining of various cell types, particularly blood cells. This staining method is named after the Russian physician Dmitri Leonidovich Romanowsky, who developed the original formulation in the late 19th century. The Romanowsky stain is a complex mixture of methylene blue, eosin, and other dyes that selectively bind to different cellular components, resulting in a distinctive color pattern that allows for the identification and differentiation of various cell types. This staining technique is particularly valuable in the analysis of blood smears, where it enables the visualization and classification of different types of blood cells, such as red blood cells, white blood cells, and platelets. Additionally, Romanowsky staining is used in the diagnosis and study of various hematological disorders, as it can provide valuable information about the morphological characteristics of blood cells, which can be indicative of underlying pathological conditions. The continued study and refinement of Romanowsky staining techniques are crucial for advancing our understanding of hematological processes and improving clinical diagnostic capabilities.
    • 5. Giemsa staining of Malaria Blood films
      Giemsa staining of malaria blood films is a crucial diagnostic technique in the identification and differentiation of malaria parasites, which are the causative agents of the infectious disease malaria. This staining method, which utilizes the Giemsa stain, a complex mixture of methylene blue, eosin, and azure dyes, allows for the visualization and characterization of the different stages of the malaria parasite's life cycle within the red blood cells of infected individuals. The distinctive color patterns created by the Giemsa stain enable the identification of specific malaria parasite species, as well as the differentiation of various developmental stages, such as trophozoites, schizonts, and gametocytes. This information is essential for accurate diagnosis, appropriate treatment, and epidemiological surveillance of malaria, a disease that remains a significant global health concern, particularly in tropical and subtropical regions. The continued study and refinement of Giemsa staining techniques for malaria diagnosis, along with the development of complementary diagnostic tools, are crucial for improving the detection and management of this infectious disease, ultimately contributing to global efforts to control and eliminate malaria.
    • 6. G-banding
      G-banding is a cytogenetic technique used for the visualization and analysis of chromosomal structures and patterns. This method involves the treatment of chromosomes with a combination of trypsin and Giemsa stain, which results in the formation of distinctive banding patterns along the length of the chromosomes. These banding patterns, known as G-bands, are unique to each chromosome and can be used to identify specific chromosomes, detect chromosomal abnormalities, and study the organization and structure of the genome. G-banding is widely used in various fields, including clinical genetics, cancer research, and evolutionary biology, as it provides a powerful tool for the identification and characterization of chromosomal aberrations, such as deletions, duplications, and translocations, which can be associated with genetic disorders, cancer, and other pathological conditions. The continued study and refinement of G-banding techniques, as well as the integration of this method with other cytogenetic and molecular techniques, are essential for advancing our understanding of the genetic basis of human health and disease, and for developing more effective diagnostic and therapeutic approaches.
    • 7. Fixatives
      Fixatives are chemical agents used in various fields of biology and medicine to preserve the structural and chemical integrity of biological samples, such as cells, tissues, and organisms, for subsequent analysis and observation. The primary function of fixatives is to prevent the degradation and alteration of the sample's morphology, composition, and molecular properties, ensuring that the sample accurately represents the in vivo state. There are a wide variety of fixatives, each with its own unique properties and applications, ranging from simple aldehydes like formaldehyde to more complex mixtures like Bouin's solution and Carnoy's fluid. The choice of fixative depends on the specific requirements of the study, the type of sample, and the desired analytical techniques. The proper selection and application of fixatives are crucial in fields such as histology, cytology, electron microscopy, and molecular biology, where the preservation of the sample's structural and chemical integrity is essential for accurate analysis and interpretation. Ongoing research into the development and optimization of fixatives, as well as the understanding of their mechanisms of action and their effects on different types of samples, is essential for advancing various areas of biological and medical research.

  • 자료후기

      Ai 리뷰
      Giemsa staining은 malaria 진단과 세포 구조 관찰에 널리 사용되는 효과적인 염색 방법으로, 핵산과 단백질을 선택적으로 염색하여 세포 내 구조를 잘 보여줍니다.

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