분자생물학기초실험/ 분자생명공학기초실험 5주차: Plasmid DNA Isolation & Restriction Digestion [A+ 리포트]

소개

연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.

목차

1. Objective
2. Materials
3. Method
4. Results & Discussion
5. Quiz
6. References

본문내용

<Results & Discussion>
The concentration of isolated plasmid DNA is measured by spectrophotometric analysis using Thermo Scientific NanoDropTM spectrophotometer. Here, the concentration of mini-prep plasmid DNA is 110.9ng/μL. In addition, the recovery and purity of isolated plasmid DNA can also be determined by spectrophotometric analysis.

<Quiz>
3. Describe the unit of restriction enzyme activity.
Enzymes are quantitated in “unit” and so by knowing the unit of restriction enzyme (RE), the amount of RE required to digest certain concentration of DNA can be determined. Hence, 1 unit indicates the amount of RE needed to digest 1μg of λ DNA in a reaction volume of 50μL in 1 hour at optimal temperature of a particular enzyme.

참고자료

· Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech. Yonsei University.
· Biolabs, New England. "Optimizing Restriction Endonuclease Reactions." Reagents For the Life Sciences Industry. Web. 25 Oct. 2016.<https://www.neb.com/protocols/2012/12/07/optimizing-restrictionendonuclease-reactions>.
· "GenElute Plasmid Miniprep Kit." SIGMA-ALORICH. SIGMA Life Science Where Bio Begins. Web. 25 Oct. 2016. <http://www.sigmaaldrich.com/content/dam/sigmaaldrich/docs/Sigma/Bulletin/pln70bul.pdf>.
· "260/280 and 260/230 Ratios." T009‐TECHNICAL BULLETIN NanoDrop 1000& 8000. Thermo Scientific. Thermo Fisher Scientific - NanoDrop Products. Web. 25 Oct. 2016. <http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf>.