노루발풀 추출물의 in vitro에서의 항산화, 항염증 및 항히스타민 활성 (In Vitro Antioxidative, Anti-inflammatory and Antihistamine Activities of Pyrola japonica Extracts)

서지정보

· 발행기관 : 한국미용학회
· 수록지 정보 : 한국미용학회지 / 18권 / 3호 / 538 ~ 544페이지
· 저자명 : 유민정, 이숙영, 차영주

초록

Pyrola japonica in Chinese traditional medicine have been used as tonics, sedatives, analgesics against rheumatoid arthritis, hemostatics,and recently isolated anti-inflammatory agents from whole plants. We have investigated antioxidant effect of P. japonica extract using DPPH raidcal scavenging test, total polyphenol and flavonoid content assay, nitrite(NO) scavenging test in cell-free system. Also,antihistamine activity by β-hexosaminidase assay and anti-inflammatory effect by inhibition rate of NO production in RAW 264.7 cell was evaluated. Skin irritation test of the extracts was evaluated in accordance with the guidelines of patch test. Plant extracts were prepared by ethanol extraction method. DPPH raidcal scavenging activity of ethanol extract of P. japonica. was the highest in 400 μg/mL as 88.62±1.51% and increased in a concentration-dependent manner. Total polyphenol and total flavonoid content of the extract was 107.5±0.03 μg/mL and 50±0.01 μg/mL, respectively. The NO scavenging activity in cell-free system was the highest in 800 μg/mL as 42.13±2.72% and it was also increased in a concentration-dependent manner. P. japonica extract will not significantly affect cell viability of RBL-2H3 cell for β-hexosaminidase assay and RAW 264.7 cell for NO content assay at the concentration of 5~50 μg/mL. The inhibition of β-hexosaminidase release was respectively indicated as 15.5, 43.0, 56.4, 67.9% in 5, 10, 20, 50 μg/mL of extract and it was increased in a concentration-dependent manner. Anti-inflammatory activity by NO content measurement in RAW cell was the highest in 50 μg/mL (NO content of 5.7±0.5 μM/mL) of P. japonica extract and additionally it was concentration-dependent. The patch application performed in order to determine dermal irritation of the extract showed result of no dermal reaction that is safety on skin. These results suggest that P. japonica extract can use as a resource of natural cosmetic material for anti-oxidant, anti-inflammatory, anti-itching gradients, and also safety on skin.

영어초록

Pyrola japonica in Chinese traditional medicine have been used as tonics, sedatives, analgesics against rheumatoid arthritis, hemostatics,and recently isolated anti-inflammatory agents from whole plants. We have investigated antioxidant effect of P. japonica extract using DPPH raidcal scavenging test, total polyphenol and flavonoid content assay, nitrite(NO) scavenging test in cell-free system. Also,antihistamine activity by β-hexosaminidase assay and anti-inflammatory effect by inhibition rate of NO production in RAW 264.7 cell was evaluated. Skin irritation test of the extracts was evaluated in accordance with the guidelines of patch test. Plant extracts were prepared by ethanol extraction method. DPPH raidcal scavenging activity of ethanol extract of P. japonica. was the highest in 400 μg/mL as 88.62±1.51% and increased in a concentration-dependent manner. Total polyphenol and total flavonoid content of the extract was 107.5±0.03 μg/mL and 50±0.01 μg/mL, respectively. The NO scavenging activity in cell-free system was the highest in 800 μg/mL as 42.13±2.72% and it was also increased in a concentration-dependent manner. P. japonica extract will not significantly affect cell viability of RBL-2H3 cell for β-hexosaminidase assay and RAW 264.7 cell for NO content assay at the concentration of 5~50 μg/mL. The inhibition of β-hexosaminidase release was respectively indicated as 15.5, 43.0, 56.4, 67.9% in 5, 10, 20, 50 μg/mL of extract and it was increased in a concentration-dependent manner. Anti-inflammatory activity by NO content measurement in RAW cell was the highest in 50 μg/mL (NO content of 5.7±0.5 μM/mL) of P. japonica extract and additionally it was concentration-dependent. The patch application performed in order to determine dermal irritation of the extract showed result of no dermal reaction that is safety on skin. These results suggest that P. japonica extract can use as a resource of natural cosmetic material for anti-oxidant, anti-inflammatory, anti-itching gradients, and also safety on skin.

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