濃度別 梔子大黃湯 肝兪穴(BL18)藥鍼이 D-galactosamine에 의해 유발된 肝損傷 白鼠에 미치는 影響 (The Effects of Herbal Acupuncture with Chijadaehwangtang Applied to the Gansu(BL18) on D-galactosamine-induced Liver Injury in Rats)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 25권 / 6호 / 95 ~ 107페이지
· 저자명 : 이혁재, 류충열, 조명래

초록

Objectives : The main purpose of this research is to observe the effects of herbal acupuncture(HA; herbal acupuncture) with Chijadaehwangtang applied to the Gansu(BL18) on D-galactosamine-induced liver injury in rats.
Methods : According to HA concentration, the experimental rats were divided 5 groups(control group, saline group, CP-1, CP-2, CP-3 group). In the control group, we first injected D-galactosamine and then didn’t treated. In the saline group, we first injected D-galactosamine and then injected saline. In the CP-1, CP-2, CP-3 group, we first injected D-galactosamine and then injected HA with Chijadaehwangtang applied to the Gansu(BL18), each 25.3mg/kg, 12.7mg/kg, 5.1mg/kg. HA with Chijadaehwangtang was treated at 20㎕ every second day, total 10 times in 20 days. We observed the changes of γ-GTP, GOT, GPT, total bilirubin, LDH, ALP, total cholesterol, triglyceride, SOD, Catalase and hepatic tissues.
Results :
1. In the changes of γ-GTP, GOT contents, as compared with control group, CP-2 group was significantly decreased.
2. In the changes of GPT content, CP-1, CP-2, CP-3 groups as compared with control group were significantly decreased.
3. In the changes of LDH content, CP-3 group as compared with control group was significantly decreased.
4. In the changes of ALP content, all groups as compared with control group were significantly decreased.
5. In the changes of SOD, Catalase contents, CP-2 group as compared with control group was significantly increased.
6. In the morphological and histopathological changes of hepatic tissues, CP-2, CP-3 groups as compared with control group were improved.
Conclusions : we observed HA with Chijadaehwangtang applied to the Gansu(BL18) can be effective treatment on hematological recovery and regenerative process in morphological liver change. Further studies about their underlying mechanism and concentrations may be needed to use HA with Chijadaehwangtang for liver injury clinically.

영어초록

Objectives : The main purpose of this research is to observe the effects of herbal acupuncture(HA; herbal acupuncture) with Chijadaehwangtang applied to the Gansu(BL18) on D-galactosamine-induced liver injury in rats.
Methods : According to HA concentration, the experimental rats were divided 5 groups(control group, saline group, CP-1, CP-2, CP-3 group). In the control group, we first injected D-galactosamine and then didn’t treated. In the saline group, we first injected D-galactosamine and then injected saline. In the CP-1, CP-2, CP-3 group, we first injected D-galactosamine and then injected HA with Chijadaehwangtang applied to the Gansu(BL18), each 25.3mg/kg, 12.7mg/kg, 5.1mg/kg. HA with Chijadaehwangtang was treated at 20㎕ every second day, total 10 times in 20 days. We observed the changes of γ-GTP, GOT, GPT, total bilirubin, LDH, ALP, total cholesterol, triglyceride, SOD, Catalase and hepatic tissues.
Results :
1. In the changes of γ-GTP, GOT contents, as compared with control group, CP-2 group was significantly decreased.
2. In the changes of GPT content, CP-1, CP-2, CP-3 groups as compared with control group were significantly decreased.
3. In the changes of LDH content, CP-3 group as compared with control group was significantly decreased.
4. In the changes of ALP content, all groups as compared with control group were significantly decreased.
5. In the changes of SOD, Catalase contents, CP-2 group as compared with control group was significantly increased.
6. In the morphological and histopathological changes of hepatic tissues, CP-2, CP-3 groups as compared with control group were improved.
Conclusions : we observed HA with Chijadaehwangtang applied to the Gansu(BL18) can be effective treatment on hematological recovery and regenerative process in morphological liver change. Further studies about their underlying mechanism and concentrations may be needed to use HA with Chijadaehwangtang for liver injury clinically.

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