Scopolamine으로 유발된 치매 白鼠모델에서 歸脾湯의 인지기능 및 기억력 향상에 미치는 효과 (The Enhancing Effects of Gwibi-tang(guīpí-tāng) on Cognitive Function and Memory in Scopolamine-induced Dementia Rat Model)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 22권 / 3호 / 1 ~ 14페이지
· 저자명 : 김정원, 김선종

초록

Objectives :The purpose of this experiment was designed to investigate the effects of Gwibi-tang(guīpí-tāng), which has been traditionally used for treatment of psychologic disease and brain damage in oriental medicine, on dementia induced by scopolamine in the rats.
Methods : Groups were divided into 4 groups: Normal group, control group, positive control group(tacrine group) and Gwibi-tang(guīpí-tāng) group. Control group :To induce dementia, 1 mg/kg of Scopolamine intraperitoneal injection is used for 14 days(1 per day) in the rats. Positive control group(tacrine group): 10 mg/kg of tacrine, the dementia treatment, is medicated in oral inoculation. After 30 mintues, 1 mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). Gwibi-tang(guīpí-tāng) group: 300 mg/kg of Gwibi-tang(guīpí-tāng) is medicated in oral inoculation. After 30 mintues, 1 mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). The present author observed the passive avoidance performance test, the spatial learning and memory ability test(Morris water maze test), the values of acetylcholine(ACh), acetylcholin esterase(AchE) activity in the hippocampal, the cerebral cortex.
Results : 1. In passive avoidance test on 1st day and 3rd day of causing dementia, Gwibi-tang(guīpí-tāng) group appeared to be more significant than positive control group. On 7th day and 14th day, Gwibi-tang(guīpí-tāng) group appeared to be more significant than normal control group and control group. However, there was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group. 2. In a test of spatial learning and memory ability, on 7th day and 14th day of causing dementia, Gwibi-tang(guīpí-tāng) group and positive control group appeared to be more significant more than control group. There was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group.
3. In Ach and AchE activity test, Ach and AchE activity in brain system and the hippocampal, cerebral cortex appeared to be more significant in Gwibi-tang(guīpí-tāng) group and positive control group than control group. However, there was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group.
4. In liver and kidney tissue tests, all experimental group appeared to be normal. which indicates that Gwibi-tang(gul̄plˊ-tāng) does not damage liver and kidney.
From the results above, Gwibi-tang(guīpí-tāng) is thought to be effective in improvement of memory related to cholinergic nerve problem and dementia.
Conclusions : The present author thought that Gwibi-tang(guīpí-tāng) might be usefully applied for prevention and treatment of dementia and memory disorder. Further study is needed to conduct a rigorous clinical research.

영어초록

Objectives :The purpose of this experiment was designed to investigate the effects of Gwibi-tang(guīpí-tāng), which has been traditionally used for treatment of psychologic disease and brain damage in oriental medicine, on dementia induced by scopolamine in the rats.
Methods : Groups were divided into 4 groups: Normal group, control group, positive control group(tacrine group) and Gwibi-tang(guīpí-tāng) group. Control group :To induce dementia, 1 mg/kg of Scopolamine intraperitoneal injection is used for 14 days(1 per day) in the rats. Positive control group(tacrine group): 10 mg/kg of tacrine, the dementia treatment, is medicated in oral inoculation. After 30 mintues, 1 mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). Gwibi-tang(guīpí-tāng) group: 300 mg/kg of Gwibi-tang(guīpí-tāng) is medicated in oral inoculation. After 30 mintues, 1 mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). The present author observed the passive avoidance performance test, the spatial learning and memory ability test(Morris water maze test), the values of acetylcholine(ACh), acetylcholin esterase(AchE) activity in the hippocampal, the cerebral cortex.
Results : 1. In passive avoidance test on 1st day and 3rd day of causing dementia, Gwibi-tang(guīpí-tāng) group appeared to be more significant than positive control group. On 7th day and 14th day, Gwibi-tang(guīpí-tāng) group appeared to be more significant than normal control group and control group. However, there was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group. 2. In a test of spatial learning and memory ability, on 7th day and 14th day of causing dementia, Gwibi-tang(guīpí-tāng) group and positive control group appeared to be more significant more than control group. There was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group.
3. In Ach and AchE activity test, Ach and AchE activity in brain system and the hippocampal, cerebral cortex appeared to be more significant in Gwibi-tang(guīpí-tāng) group and positive control group than control group. However, there was no significant difference between Gwibi-tang(guīpí-tāng) group and positive control group.
4. In liver and kidney tissue tests, all experimental group appeared to be normal. which indicates that Gwibi-tang(gul̄plˊ-tāng) does not damage liver and kidney.
From the results above, Gwibi-tang(guīpí-tāng) is thought to be effective in improvement of memory related to cholinergic nerve problem and dementia.
Conclusions : The present author thought that Gwibi-tang(guīpí-tāng) might be usefully applied for prevention and treatment of dementia and memory disorder. Further study is needed to conduct a rigorous clinical research.

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