육미지황탕의 만성질환 치료 및 약물대사 관련 신호에 미치는 영향 (The effect of Yuk-Mi-Jihwang tang on the chronic disease and the metabolic signal pathways)

서지정보

· 발행기관 : 대한한의학방제학회
· 수록지 정보 : 대한한의학 방제학회지 / 33권 / 2호 / 139 ~ 147페이지
· 저자명 : 박효기, 박선빈, 이종록, 박숙자, 박선동

초록

Objectives : The liver plays an important role in maintaining homeostasis and overall health in the body. Traditional herbal prescriptions have been widely utilized as therapeutic strategies for various diseases in the organs including the liver.
Methods : This study investigates the protective effects of Yuk-Mi-Jihwang tang (YMJ) against oxidative stress in hepatocytes and highlights its association with AMP activated kinase (AMPK) pathway activation, which was assessed by immunoblot, flow cytometry and cell viability assays.
Results : YMJ significantly increased cell viability, confirming its protective effects against oxidative stress-induced cell death. The prescription showed ROS-scavenging activity and protected mitochondrial membrane potential, as demonstrated by flow cytometry. YMJ also activated AMPK, leading to the phosphorylation of ACC, indicating that YMJ's effects are mediated through the AMPK pathway.
Conclusion : These results suggest the potential of YMJ as an antioxidant therapy, which serves as a molecular approach for treating liver diseases.

영어초록

Objectives : The liver plays an important role in maintaining homeostasis and overall health in the body. Traditional herbal prescriptions have been widely utilized as therapeutic strategies for various diseases in the organs including the liver.
Methods : This study investigates the protective effects of Yuk-Mi-Jihwang tang (YMJ) against oxidative stress in hepatocytes and highlights its association with AMP activated kinase (AMPK) pathway activation, which was assessed by immunoblot, flow cytometry and cell viability assays.
Results : YMJ significantly increased cell viability, confirming its protective effects against oxidative stress-induced cell death. The prescription showed ROS-scavenging activity and protected mitochondrial membrane potential, as demonstrated by flow cytometry. YMJ also activated AMPK, leading to the phosphorylation of ACC, indicating that YMJ's effects are mediated through the AMPK pathway.
Conclusion : These results suggest the potential of YMJ as an antioxidant therapy, which serves as a molecular approach for treating liver diseases.

참고자료

· 없음