黃連解毒湯, 黃連, 黃芩, 黃柏, 梔子의 Staphylococcus aureus에 대한 항균효과 (Antimicrobial Activity of Hwangryunheadok-tang, and Scutellariae Radix Extract, Phellodendri Cortex Extract, Coptis Rhizoma Extract, Gardenia Jasminoides Extract against Staphylococcus aureus)

서지정보

· 발행기관 : 대한한방내과학회
· 수록지 정보 : 대한한방내과학회지 / 35권 / 4호 / 455 ~ 471페이지
· 저자명 : 김인수, 오현승, 신민구, 이영수

초록

Objectives: This experiment was done for investigating antimicrobial activity of Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, Gardenia Jasminoides extract against Staphylococcus aureus.
Methods: After administering S. aureus on a bacterial culture media plate, antimicrobial activity was tested by dripping 80 μl diluted Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, and Gardenia Jasminoides extract (100%, 50%, 10%, 1%) on plates that were cultivated for a span of time from 16 to 72 hours. Also, minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution density solution that has antimicrobial activity between 80 μl and 20 μl (80 μl, 60 μl, 40 μl, 20 μl) in measure of density. Antimicrobial activity of Hwangryunheadok-tang and four kinds of extracts against S. aureus was continually displayed.
Results:1. S. aureus (Standard Microorganism, ATCC)(1) Antimicrobial activity was displayed for Hwangryunheadok-tang, Scutellariae Radix extract, and Phellodendri Cortex extract respectively in the undiluted solution and 50% of diluted magnification. Gardenia Jasminoides extract showed its activity only in the undiluted solution; Coptis Rhizoma extract showed its activity down to 10% of diluted magnification. The antimicrobial activity of the undiluted solution was increased when the volume of inoculation increased. But, there was no difference when time was extended for cultivation.
(2) MIC of Hwangryunheadok-tang, Scutellariae Radix extract was 50%, 20 μl. Coptis Rhizoma extract was 10%, 20 μl, Phellodendri Cortex extract was 50%, 80 μl and Gardenia Jasminoides extract was 100%, 60 μl.
2. S. aureus isolated from diarrheal patients(1) When compared to standard microorganism, MIC has decreased. As a result, their antimicrobial activity has increased.
(2) Antimicrobial activity of Hwangryunheadok-tang and four extracts was continually shown in extending of the time, 16, 24 and 72 hours.
Conclusions: The author comes to the conclusion that Hwangryunheadok-tang, and four kinds of extracts have antimicrobial activity against S. aureus. Especially, when comparing standard microorganisms with S. aureus isolated from diarrheal patients, some cases showed that antimicrobial activity of all the extracts were better than antibiotics. Thus, if a further study is performed, the use of Hwangryunheadok-tang, and four kinds of extracts will be valuable and beneficial in clinical treatments.

영어초록

Objectives: This experiment was done for investigating antimicrobial activity of Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, Gardenia Jasminoides extract against Staphylococcus aureus.
Methods: After administering S. aureus on a bacterial culture media plate, antimicrobial activity was tested by dripping 80 μl diluted Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, and Gardenia Jasminoides extract (100%, 50%, 10%, 1%) on plates that were cultivated for a span of time from 16 to 72 hours. Also, minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution density solution that has antimicrobial activity between 80 μl and 20 μl (80 μl, 60 μl, 40 μl, 20 μl) in measure of density. Antimicrobial activity of Hwangryunheadok-tang and four kinds of extracts against S. aureus was continually displayed.
Results:1. S. aureus (Standard Microorganism, ATCC)(1) Antimicrobial activity was displayed for Hwangryunheadok-tang, Scutellariae Radix extract, and Phellodendri Cortex extract respectively in the undiluted solution and 50% of diluted magnification. Gardenia Jasminoides extract showed its activity only in the undiluted solution; Coptis Rhizoma extract showed its activity down to 10% of diluted magnification. The antimicrobial activity of the undiluted solution was increased when the volume of inoculation increased. But, there was no difference when time was extended for cultivation.
(2) MIC of Hwangryunheadok-tang, Scutellariae Radix extract was 50%, 20 μl. Coptis Rhizoma extract was 10%, 20 μl, Phellodendri Cortex extract was 50%, 80 μl and Gardenia Jasminoides extract was 100%, 60 μl.
2. S. aureus isolated from diarrheal patients(1) When compared to standard microorganism, MIC has decreased. As a result, their antimicrobial activity has increased.
(2) Antimicrobial activity of Hwangryunheadok-tang and four extracts was continually shown in extending of the time, 16, 24 and 72 hours.
Conclusions: The author comes to the conclusion that Hwangryunheadok-tang, and four kinds of extracts have antimicrobial activity against S. aureus. Especially, when comparing standard microorganisms with S. aureus isolated from diarrheal patients, some cases showed that antimicrobial activity of all the extracts were better than antibiotics. Thus, if a further study is performed, the use of Hwangryunheadok-tang, and four kinds of extracts will be valuable and beneficial in clinical treatments.

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