고지방식이 섭식 흰쥐의 간조직에 미치는 한방초콩환의 영향 (Effect of Herbal Chokong Pill on the Microstructure of Liver Cell in Rats Fed High Fat Diet)

서지정보

· 발행기관 : 한국생명과학회
· 수록지 정보 : 생명과학회지 / 18권 / 2호 / 200 ~ 205페이지
· 저자명 : 박찬성, 김동한, 김미림

초록

The purpose of this study is to investigate the effects of the herbal Chokong pill (hereafter HCKP) on the liver cell and enzyme activities of serum in rat. HCKP were mixed with pickled black soybeans and five different kinds of medicinal herbs (Rhynchosia nulubilis, Glycyrrhiza uralensis, Zizyphus vulgaris, Atractylodes macrocephala K., Astragalus membranaceus and Cornus officinalis). Four groups of male Sprague-Dawley rats were fed by different diets for 9 weeks: normal diet (Nor), high-fat diet (HF), high-fat diet supplemented with 1% (T1) and 5% (T5) HCKP powder, respectively. Depending on the presence of HCKP in high fat diet, the activities of the blood serum GOT and GPT were decreased. GOT and GPT activities of T1 and T5 were decreased 6.1%, 17.8% and 25.4%, 32.4% compared with HF. On microstructure observing through the transmission electron microscope (TEM) of liver cell, in normal group, a normal large and clear nucleus, rough endoplasmic reticulum (RER) and mitochondria possessing well-defined double outer-limiting membranes were found. However, in HF, it was hard to observe the microstructures in cytoplasm, because of too many fat granules. It showed severely damaged cell, pyknotic nucleus, swollen disintegrating RER and mitochontria loosing the cristae. In T1, there were more repaired liver cells and less fat granules than HF. In T5, there were much less numbers and smaller size of the fat granules than T1, and the morphology was similar to normal cell.

영어초록

The purpose of this study is to investigate the effects of the herbal Chokong pill (hereafter HCKP) on the liver cell and enzyme activities of serum in rat. HCKP were mixed with pickled black soybeans and five different kinds of medicinal herbs (Rhynchosia nulubilis, Glycyrrhiza uralensis, Zizyphus vulgaris, Atractylodes macrocephala K., Astragalus membranaceus and Cornus officinalis). Four groups of male Sprague-Dawley rats were fed by different diets for 9 weeks: normal diet (Nor), high-fat diet (HF), high-fat diet supplemented with 1% (T1) and 5% (T5) HCKP powder, respectively. Depending on the presence of HCKP in high fat diet, the activities of the blood serum GOT and GPT were decreased. GOT and GPT activities of T1 and T5 were decreased 6.1%, 17.8% and 25.4%, 32.4% compared with HF. On microstructure observing through the transmission electron microscope (TEM) of liver cell, in normal group, a normal large and clear nucleus, rough endoplasmic reticulum (RER) and mitochondria possessing well-defined double outer-limiting membranes were found. However, in HF, it was hard to observe the microstructures in cytoplasm, because of too many fat granules. It showed severely damaged cell, pyknotic nucleus, swollen disintegrating RER and mitochontria loosing the cristae. In T1, there were more repaired liver cells and less fat granules than HF. In T5, there were much less numbers and smaller size of the fat granules than T1, and the morphology was similar to normal cell.

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