健側 取穴 多種 鍼刺法이 白鼠의 神經病理性 疼痛에 미치는 영향 (Effects of Acupuncture, Electro-acupuncture, Low Level He-Ne Laser Therapy at Oe-gwan(TE5) ․ Chogimup (GB41) on L5 Spinal Nerve Ligation Model in Rats)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 24권 / 5호 / 137 ~ 150페이지
· 저자명 : 정정희, 조명래, 위통순, 류충열

초록

Objectives : To find effects of acupuncture, electro-acupuncture, low level He-Ne laser therapy(LLLT) at TE5, GB41 in the neuropathic pain. We made experiment on rats ligated L5 spinal nerve like general herniation of nucleus pulposus(HNP).
Methods : A model of neuropathic pain was made by isolating Left 5th lumbar spinal nerve of rats. Three days after the neuropathic surgery, acupuncture and LLLT, electro-acupuncture was injected at TE5, GB41 one time a day for a week. Each group was divided two. one is opposite side performed the surgery which is right, another is left side performed the surgery. After that, the author examined the withdrawal response of neuropathic rats’ legs by von Frey filament and acetone stimulation. And also the author examined c-Fos, Nociceptin and KOR-3 in the midbrain central gray of neuropathic rats.
Results : As we have observed the effect of mechanical allodynia, LT-R group were diminished on 6th day compared with control group, EA-L group, EA-R group and LT-L group were diminished on 7th day compared with control group.
As we have observed the effect of cold allodynia, EA-R group were diminished on 6th day, 7th day compared with control group.
As we have observed the effect of activity of c-Fos in the central gray part, EA-R group and LT-R group were diminished compared with control group.
As we have observed the effect of activity of Nociceptin in the central gray part, EA-R group were a little increased compared with control group but it is not reliability.
As we have observed the effect of activity of KOR-3 in the central gray part, EA-R group were significantly increased compared with control group.
Conclusions : We have noticed that effect of acupuncture at opposite side of sickness and powerful stimulation could be more effective, because of EA-R group have more controllable effect all test we have done on the other hand EA-L group have only effect on mechanical allodynia. This study can be used in clinical therapy for neuropathic pain. But it is not reliability that Nociceptin have effectively to control pain. Therefore We have to follow up about that.
Key Words : Neuropathic pain, TE5, GB41, low level He-Ne laser therapy, c-Fos, Nociceptin, KOR-3, electro-acupuncture, mechanical allodynia, cold allodynia

영어초록

Objectives : To find effects of acupuncture, electro-acupuncture, low level He-Ne laser therapy(LLLT) at TE5, GB41 in the neuropathic pain. We made experiment on rats ligated L5 spinal nerve like general herniation of nucleus pulposus(HNP).
Methods : A model of neuropathic pain was made by isolating Left 5th lumbar spinal nerve of rats. Three days after the neuropathic surgery, acupuncture and LLLT, electro-acupuncture was injected at TE5, GB41 one time a day for a week. Each group was divided two. one is opposite side performed the surgery which is right, another is left side performed the surgery. After that, the author examined the withdrawal response of neuropathic rats’ legs by von Frey filament and acetone stimulation. And also the author examined c-Fos, Nociceptin and KOR-3 in the midbrain central gray of neuropathic rats.
Results : As we have observed the effect of mechanical allodynia, LT-R group were diminished on 6th day compared with control group, EA-L group, EA-R group and LT-L group were diminished on 7th day compared with control group.
As we have observed the effect of cold allodynia, EA-R group were diminished on 6th day, 7th day compared with control group.
As we have observed the effect of activity of c-Fos in the central gray part, EA-R group and LT-R group were diminished compared with control group.
As we have observed the effect of activity of Nociceptin in the central gray part, EA-R group were a little increased compared with control group but it is not reliability.
As we have observed the effect of activity of KOR-3 in the central gray part, EA-R group were significantly increased compared with control group.
Conclusions : We have noticed that effect of acupuncture at opposite side of sickness and powerful stimulation could be more effective, because of EA-R group have more controllable effect all test we have done on the other hand EA-L group have only effect on mechanical allodynia. This study can be used in clinical therapy for neuropathic pain. But it is not reliability that Nociceptin have effectively to control pain. Therefore We have to follow up about that.
Key Words : Neuropathic pain, TE5, GB41, low level He-Ne laser therapy, c-Fos, Nociceptin, KOR-3, electro-acupuncture, mechanical allodynia, cold allodynia

참고자료

· 없음