혈관 비틀림이 백서 천층하복부 동맥의 초미세문합에 미치는 효과 (Twisting Effect on Supermicroanastomosis of the Superficial Inferior Epigastric Artery in a Rat Model)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 33권 / 5호 / 375 ~ 384페이지
· 저자명 : 서미현, 김성민, 어미영, 강지영, 명훈, 이종호

초록

Purpose: The advent of microsurgical technique and instruments, particularly in the field of perforator flap and supermicrosurgery,which have expanded the scope of microsurgery. However, supermicroanastomosis without any compression,tension, or distortions must be achieved to reach successful outcomes. Small-caliber vessels, such as those with an internal diameter less than 0.2 mm, are susceptible to inadvertent twisting of the anastomosis. In this study, using the superficial inferior epigastric artery (SIEA)-based flap model in Sprague-Dawley (SD) rats, we evaluated the acceptable limits of twisting effects on supermicroanastomotic sites.
Methods: A total of 20 supermicroanastomoses were performed using the SIEA-based flap model in 10 male SD rats,10-weeks-of-age, weighing 300∼350 g. Rats were divided into five groups of two with four flaps as follows: 1) sham, 2)control group with end to end SIEA arterial supermicroanastomosis, 3) experimental I (EA1) with 90o twisting, 4) experimental II (EA2) with 180o twisting, and 5) experimental III (EA3) with 270o twisting of the supermicroanastomosis. Each SIEA was anastomosed using six 11-0 EthilonⓇ (Ethicon Inc. Co., NJ, USA) stitches except in the sham group where the SIEA was only clamped with Supermicro vascular clampsⓇ (S&T, Neuhausen, Switzerland) for 20 minutes.
Results: The anastomosed arterial patency showed no remarkable changes according to doppler waveforms measured with a Smardop 45 Doppler System (Hadeco Inc., Kawasaki, Japan). The pulsatility index (PI) was increased at postoperative day 10 in the EA2 and EA3 groups, and the resistance index (RI) showed no statistically significant difference between preoperative and postoperative values at 10 days. Histologic specimens from the EA3 group showed increased tunica media necrosis,convolution of the internal elastic lamina, densely packed platelets, fibrin, and erythrocytes. Flap viability and anastomosed vessel patency were not significantly affected by the degree of arterial twisting in this study, other than in the EA3 group where minor effects on arterial patency of the microanastomoses were encountered.
Conclusion: It appears that minor twisting on small caliber arteries, used in supermicroanastomoses, can be tolerated. However,twisting should be avoided as much as possible, and more than 180o twisting must be prevented in clinical practice.

영어초록

Purpose: The advent of microsurgical technique and instruments, particularly in the field of perforator flap and supermicrosurgery,which have expanded the scope of microsurgery. However, supermicroanastomosis without any compression,tension, or distortions must be achieved to reach successful outcomes. Small-caliber vessels, such as those with an internal diameter less than 0.2 mm, are susceptible to inadvertent twisting of the anastomosis. In this study, using the superficial inferior epigastric artery (SIEA)-based flap model in Sprague-Dawley (SD) rats, we evaluated the acceptable limits of twisting effects on supermicroanastomotic sites.
Methods: A total of 20 supermicroanastomoses were performed using the SIEA-based flap model in 10 male SD rats,10-weeks-of-age, weighing 300∼350 g. Rats were divided into five groups of two with four flaps as follows: 1) sham, 2)control group with end to end SIEA arterial supermicroanastomosis, 3) experimental I (EA1) with 90o twisting, 4) experimental II (EA2) with 180o twisting, and 5) experimental III (EA3) with 270o twisting of the supermicroanastomosis. Each SIEA was anastomosed using six 11-0 EthilonⓇ (Ethicon Inc. Co., NJ, USA) stitches except in the sham group where the SIEA was only clamped with Supermicro vascular clampsⓇ (S&T, Neuhausen, Switzerland) for 20 minutes.
Results: The anastomosed arterial patency showed no remarkable changes according to doppler waveforms measured with a Smardop 45 Doppler System (Hadeco Inc., Kawasaki, Japan). The pulsatility index (PI) was increased at postoperative day 10 in the EA2 and EA3 groups, and the resistance index (RI) showed no statistically significant difference between preoperative and postoperative values at 10 days. Histologic specimens from the EA3 group showed increased tunica media necrosis,convolution of the internal elastic lamina, densely packed platelets, fibrin, and erythrocytes. Flap viability and anastomosed vessel patency were not significantly affected by the degree of arterial twisting in this study, other than in the EA3 group where minor effects on arterial patency of the microanastomoses were encountered.
Conclusion: It appears that minor twisting on small caliber arteries, used in supermicroanastomoses, can be tolerated. However,twisting should be avoided as much as possible, and more than 180o twisting must be prevented in clinical practice.

참고자료

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