Fluconazole 첨가 사브로덱스트로오스 한천 배지를 사용한 Candida 종의 Fluconazole 내성 검색 (Screening of Fluconazole-Resistant Candida Species with Fluconazole-Containing Sabouraud Dextrose Agar Plates)

서지정보

· 발행기관 : 대한의진균학회
· 수록지 정보 : 대한의진균학회지 / 14권 / 1호 / 16 ~ 22페이지
· 저자명 : 이미경, 김태형

초록

Background: Antifungal susceptibility testing methods are relatively expensive and laborious and are not easily applicable for screening purposes in routine laboratories.
Objective: We have developed a susceptibility screening method by adding fluconazole to Sabouraud dextrose agar (SDA) plate. In this study, SDA plates screening was compared with the Clinical and Laboratory Standards Institutes (CLSI) broth microdilution method for determining fluconazole
susceptibility of Candida spp.
Methods: A total 186 isolates of Candida spp. (134 C. albicans, 24 C. tropicalis, 15 C. glabrata, 13 C. parapsilosis) were tested with the CLSI document M27-A2 method and fluconazole-containing SDA plate (0, 8, 16 μg/mL of fluconazole). By this method, colony count and diameter were read after 24 hr of incubation.
Results: Fluconazole-susceptible Candida spp. are significantly smaller (≤50%) on SDA with fluconazole than on fluconazole-free SDA. On SDA with 8 and 16 μg of fluconazole per mL, 80.7% (150/186) and 87.1% (162/186) of isolates were correctly predicted, respectively.
Conclusions: These data suggest that the fluconazole-containing SDA plates can be used as a routine screening procedure for susceptibility of Candida spp. in clinical laboratories.

영어초록

Background: Antifungal susceptibility testing methods are relatively expensive and laborious and are not easily applicable for screening purposes in routine laboratories.
Objective: We have developed a susceptibility screening method by adding fluconazole to Sabouraud dextrose agar (SDA) plate. In this study, SDA plates screening was compared with the Clinical and Laboratory Standards Institutes (CLSI) broth microdilution method for determining fluconazole
susceptibility of Candida spp.
Methods: A total 186 isolates of Candida spp. (134 C. albicans, 24 C. tropicalis, 15 C. glabrata, 13 C. parapsilosis) were tested with the CLSI document M27-A2 method and fluconazole-containing SDA plate (0, 8, 16 μg/mL of fluconazole). By this method, colony count and diameter were read after 24 hr of incubation.
Results: Fluconazole-susceptible Candida spp. are significantly smaller (≤50%) on SDA with fluconazole than on fluconazole-free SDA. On SDA with 8 and 16 μg of fluconazole per mL, 80.7% (150/186) and 87.1% (162/186) of isolates were correctly predicted, respectively.
Conclusions: These data suggest that the fluconazole-containing SDA plates can be used as a routine screening procedure for susceptibility of Candida spp. in clinical laboratories.

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