참당귀 추출물의 청색광 유도 망막색소상피세포(ARPE-19) 손상으로부터의 보호 효과 (Protective Effect of Angelica gigas Nakai Extract Against Blue Light-induced Cell Death on Human Retinal Pigmented Epithelium (ARPE-19) Cell Viability)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 66권 / 3호 / 146 ~ 151페이지
· 저자명 : 김현우, 이윤제, 강현본, 김형회, 송일대, 강재선

초록

Light pollution such as blue light exposure can damage the visual photoreceptors and lead to cell death anddegenerative retinal diseases. Despite the increasing number of patients suffering from retinal disease, the therapeutic agentsare still insufficient. Angelica gigas Nakai extract (AGNEX) containing a high proportion of decursin derivatives haseffectively protected cells under oxidative stress or high-level blue light. However, it remains unclear whether it protectsretinal cells effectively. Here, we investigated the cytoprotective effect of AGNEX on retinal cells damaged using reactiveoxygen species (ROS), which decreased the viability of retinal cells by causing oxidative stress. The cell viability with 600μM hydrogen peroxide treatment was less than 70% of the normal control group. However, the group treated with 40 μMof AGNEX showed recovery as the normal control group. ROS levels were decreased in the AGNEX treated at 10-20μM compared with the 600 μM hydrogen peroxide group. The AGNEX treated group was reduced by 20% comparedwith the untreated control group. This study suggests that AGNEX could prevent damage to blue light-induced retinal cellsby oxidative stress relief, suggesting its potential as a therapeutic agent similar to eye care functional foods and asmedicine against retinal cell damage.

영어초록

Light pollution such as blue light exposure can damage the visual photoreceptors and lead to cell death anddegenerative retinal diseases. Despite the increasing number of patients suffering from retinal disease, the therapeutic agentsare still insufficient. Angelica gigas Nakai extract (AGNEX) containing a high proportion of decursin derivatives haseffectively protected cells under oxidative stress or high-level blue light. However, it remains unclear whether it protectsretinal cells effectively. Here, we investigated the cytoprotective effect of AGNEX on retinal cells damaged using reactiveoxygen species (ROS), which decreased the viability of retinal cells by causing oxidative stress. The cell viability with 600μM hydrogen peroxide treatment was less than 70% of the normal control group. However, the group treated with 40 μMof AGNEX showed recovery as the normal control group. ROS levels were decreased in the AGNEX treated at 10-20μM compared with the 600 μM hydrogen peroxide group. The AGNEX treated group was reduced by 20% comparedwith the untreated control group. This study suggests that AGNEX could prevent damage to blue light-induced retinal cellsby oxidative stress relief, suggesting its potential as a therapeutic agent similar to eye care functional foods and asmedicine against retinal cell damage.

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