혈관생성 억제제를 주사한 마우스 모델에서의 골수 세포의 복강 내 주입 후 생착 (Engraftment of Intraperitoneally Injected Bone Marrow Cells to Newborn Mice Injected with an Angiogenesis Inhibitor)

서지정보

· 발행기관 : 대한신생아학회
· 수록지 정보 : Neonatal medicine / 15권 / 1호 / 22 ~ 31페이지
· 저자명 : 조수진, 주선영, 우소연, 강형진, 안효섭, 유경하, 박은애

초록

Purpose : Bronchopulmonary dysplasia (BPD) is characterized by arrested vascular and
alveolar growth in the premature lung. Considering the consequences of arrested lung growth,
the idea of administering bone marrow cells to enhance the inborn repair mechanism is
promising as this may reduce the morbidity and mortality of BPD. We followed enhanced
green fluorescent protein (EGFP)-labeled bone marrow cells (BMC) injected intraperitoneally
into non-EGFP mice in order to determine their fate after transplantation.
Methods : An angiogenesis inhibitor, SU1498, was injected subcutaneously on day 3 in
non-EGFP C57BL/6 newborn mice to create a model of arrested alveolar development. On
the following day, 1×106 BMCs isolated from major histocompatibility complex (MHC)-
matched syngenic EGFP mice were injected intraperitoneally to non-EGFP BPD mice. Morphometric
analysis, immunostaining, and confocal microscopy were performed to determine
the fate of EGFP-positive stem cells in the injured lung.
Results : SU1498 injection reduced alveolar surface area and mean alveolar volume in
newborn mice. BMC injection resulted in recovery of lung structure comparable to controls.
EGFP-positive BMCs were identified in the lungs of the recipient mice after intraperitoneal
injection. The injected EGFP cells were co-stained with endothelial and epithelial cells of
the developing lung as determined by confocal microscopy.
Conclusion : Our results illustrated that EGFP-positive BMCs engrafted and transdifferentiated
into epithelial and endothelial cells after intraperitoneal injection in a mouse
model of arrested alveolar development.

영어초록

Purpose : Bronchopulmonary dysplasia (BPD) is characterized by arrested vascular and
alveolar growth in the premature lung. Considering the consequences of arrested lung growth,
the idea of administering bone marrow cells to enhance the inborn repair mechanism is
promising as this may reduce the morbidity and mortality of BPD. We followed enhanced
green fluorescent protein (EGFP)-labeled bone marrow cells (BMC) injected intraperitoneally
into non-EGFP mice in order to determine their fate after transplantation.
Methods : An angiogenesis inhibitor, SU1498, was injected subcutaneously on day 3 in
non-EGFP C57BL/6 newborn mice to create a model of arrested alveolar development. On
the following day, 1×106 BMCs isolated from major histocompatibility complex (MHC)-
matched syngenic EGFP mice were injected intraperitoneally to non-EGFP BPD mice. Morphometric
analysis, immunostaining, and confocal microscopy were performed to determine
the fate of EGFP-positive stem cells in the injured lung.
Results : SU1498 injection reduced alveolar surface area and mean alveolar volume in
newborn mice. BMC injection resulted in recovery of lung structure comparable to controls.
EGFP-positive BMCs were identified in the lungs of the recipient mice after intraperitoneal
injection. The injected EGFP cells were co-stained with endothelial and epithelial cells of
the developing lung as determined by confocal microscopy.
Conclusion : Our results illustrated that EGFP-positive BMCs engrafted and transdifferentiated
into epithelial and endothelial cells after intraperitoneal injection in a mouse
model of arrested alveolar development.

참고자료

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