물집유사천포창, 보통천포창 및 낙엽천포창에서 Matrix Metalloproteinase-2, -3, -9의 발현에 관한 연구 (Matrix Metalloproteinases and Autoimmune Bulbous Diseases: Expression of MMP-2, -3, and -9 in Bullous Pemphigoid, Pemphigus Vulgaris, and Pemphigus Foliaceus)

서지정보

· 발행기관 : 대한피부과학회
· 수록지 정보 : 대한피부과학회지 / 47권 / 11호 / 1261 ~ 1266페이지
· 저자명 : 전지선, 윤숙정, 김성진, 이승철, 원영호, 이지범

초록

Background: Matrix metalloproteinases (MMPs) participate in extracellular matrix degradation and may play an
important role in basal membrane damage in many dermatologic diseases. Recent studies implicated the importance
of MMP-9 in the pathogenesis of bulla formation of bullous pemphigoid (BP). Various autoimmune bullous diseases
are strongly associated with desmosome or hemidesmosome pathologies, and show an increased level of lesional
MMP and exposed autoantigens from these structures.
Objective: This study evaluated the level of MMP-2, -3, and -9 in three types of autoimmune bullous disease [BP,
pemphigus vulgaris (PV), pemphigus foliaceus (PF)] with the aim of investigating the role of MMPs in the
pathogenesis of autoimmune bullous diseases.
Methods: Sample specimens were obtained from skin lesions of patients with BP (n=12), PV (n=10), and PF
(n=12), and from normal controls (n=8). The immunohistochemical expression of MMP-2, -3, and -9 was analyzed
and serum levels of MMP-2, -3, and -9 were measured by enzyme-linked immunosorbant assay (ELISA). The results
were analyzed with reference to graded levels of clinical severity.
Results: Expression of dermal MMP-2, -3, and -9 were increased in BP, PV, and PF (p=0.036, 0.022, and 0.015,
respectively). However, decreased expression of the three MMPs in the epidermis of skin lesions may have resulted
from epidermal destruction. ELISA-determined serum levels of MMP-2, -3, and -9 increased in BP, PV and PF.
Interestingly, MMP-2 was significantly increased in the sera of BP patients (p=0.015), consistent with the previous
studies concerning the role of gelatinase (MMP-2 and -9) in the pathogenesis of BP. In BP patients, clinical severity
was proportional to increased levels of MMP-2 in both skin lesions and and sera.
Conclusion: The increased expression of MMP-2, -3, and -9 in skin lesions and sera may reflect the involvement
of these enzymes in the mechanism of bulla formation in autoimmune bullous diseases including BP. In addition,
expression of MMP and clinical severity may be closely connected.

영어초록

Background: Matrix metalloproteinases (MMPs) participate in extracellular matrix degradation and may play an
important role in basal membrane damage in many dermatologic diseases. Recent studies implicated the importance
of MMP-9 in the pathogenesis of bulla formation of bullous pemphigoid (BP). Various autoimmune bullous diseases
are strongly associated with desmosome or hemidesmosome pathologies, and show an increased level of lesional
MMP and exposed autoantigens from these structures.
Objective: This study evaluated the level of MMP-2, -3, and -9 in three types of autoimmune bullous disease [BP,
pemphigus vulgaris (PV), pemphigus foliaceus (PF)] with the aim of investigating the role of MMPs in the
pathogenesis of autoimmune bullous diseases.
Methods: Sample specimens were obtained from skin lesions of patients with BP (n=12), PV (n=10), and PF
(n=12), and from normal controls (n=8). The immunohistochemical expression of MMP-2, -3, and -9 was analyzed
and serum levels of MMP-2, -3, and -9 were measured by enzyme-linked immunosorbant assay (ELISA). The results
were analyzed with reference to graded levels of clinical severity.
Results: Expression of dermal MMP-2, -3, and -9 were increased in BP, PV, and PF (p=0.036, 0.022, and 0.015,
respectively). However, decreased expression of the three MMPs in the epidermis of skin lesions may have resulted
from epidermal destruction. ELISA-determined serum levels of MMP-2, -3, and -9 increased in BP, PV and PF.
Interestingly, MMP-2 was significantly increased in the sera of BP patients (p=0.015), consistent with the previous
studies concerning the role of gelatinase (MMP-2 and -9) in the pathogenesis of BP. In BP patients, clinical severity
was proportional to increased levels of MMP-2 in both skin lesions and and sera.
Conclusion: The increased expression of MMP-2, -3, and -9 in skin lesions and sera may reflect the involvement
of these enzymes in the mechanism of bulla formation in autoimmune bullous diseases including BP. In addition,
expression of MMP and clinical severity may be closely connected.

참고자료

· 없음