고강도의 저항성 운동 수행 시 흰쥐 Type II 골격근에서 노화에 의한 근합성 신호 변화 (Change of protein synthesis signaling with an acute intensive resistance exercise in Type II skeletal muscle of aged rats)

서지정보

· 발행기관 : 한국체육과학회
· 수록지 정보 : 한국체육과학회지 / 21권 / 5호 / 983 ~ 995페이지
· 저자명 : 권형태, 김창근, 김효정

초록

The present study was performed to investigate the effect of resistance exercise on aged muscle protein synthesis. Young (12 wk, n=28) and aged (100 wk, n=26) male, Sprague-Dawley rats (n = 56) were randomly assigned to the time course of resistance exercise. Ladder climbing was performed with a 1 m wire ladder, at an 85° angle and the weight bearing was added to the tail (1RM 60-80%, total 25-30 climbs/h). Muscle samples were obtained before and recovery period of the exercise (pre, immediately after, recovery 1hr, and 3hr from the climbing). Protein expressions were analyzed for PKB/Akt, mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6k), eukaryotic initiation factor (eIF) 4E-binding protein (4E-BP1) and AMPK phosphorylation. This type of resistance exercise generated a significant increase in mTOR phosphorylation immediately after exercise in aged rats (∼71%, p=.02). In addition, 4EBP1 phosphorylation was significantly decreased at the immediately (∼42%, p=.05) after exercise and decreased up to an hour recovery. However, Phosphorylation of p70S6K was elevated above rest level throughout the 3hrs recovery (347%, p=.004). On the other hand, Phospho-AMPK (Thr172) tended to be greater than the young rats. These results suggest that the intensitive resistance exercise positively effect on muscle protein synthesis in aged skeletal muscle.

영어초록

The present study was performed to investigate the effect of resistance exercise on aged muscle protein synthesis. Young (12 wk, n=28) and aged (100 wk, n=26) male, Sprague-Dawley rats (n = 56) were randomly assigned to the time course of resistance exercise. Ladder climbing was performed with a 1 m wire ladder, at an 85° angle and the weight bearing was added to the tail (1RM 60-80%, total 25-30 climbs/h). Muscle samples were obtained before and recovery period of the exercise (pre, immediately after, recovery 1hr, and 3hr from the climbing). Protein expressions were analyzed for PKB/Akt, mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6k), eukaryotic initiation factor (eIF) 4E-binding protein (4E-BP1) and AMPK phosphorylation. This type of resistance exercise generated a significant increase in mTOR phosphorylation immediately after exercise in aged rats (∼71%, p=.02). In addition, 4EBP1 phosphorylation was significantly decreased at the immediately (∼42%, p=.05) after exercise and decreased up to an hour recovery. However, Phosphorylation of p70S6K was elevated above rest level throughout the 3hrs recovery (347%, p=.004). On the other hand, Phospho-AMPK (Thr172) tended to be greater than the young rats. These results suggest that the intensitive resistance exercise positively effect on muscle protein synthesis in aged skeletal muscle.

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