신생쥐에서 저산소성 허혈성 뇌손상 후 흑색질-선조체 및 운동행위 손상 (Hypoxia-Ischemia Induced Nigrostriatal System Damages and Motor Behavioral Changes in Neonatal Rat Brain)

서지정보

· 발행기관 : 대한재활의학회
· 수록지 정보 : Annals of Rehabilitation Medicine / 33권 / 6호 / 649 ~ 656페이지
· 저자명 : 김세원, 김승범, 이수영, 김종문, 임정훈, 이인식, 이종민, 고성은

초록

Objective: To investigate hypoxia-ischemia induced nigrostriatal system damages and motor behavioral changes in the immature developing rat brain.
Method: For establishment of hypoxia-ischemia (HI) injury, bilateral common carotid artery occlusion was performed permanently with bipolar electrocoagulation in the postnatal day 5 rats. And then rat pups were immediately subjected to hypoxic exposure (8% oxygen) at 37℃ for 1 hour. The control group underwent sham operation and normoxic exposure. Brain injury including striatonigral system was examined. Motor behavioral changes were investigated at 2-, 4-, 6- and 8-week after HI injury using the Rota-rod test and the d-amphetamine-induced locomotor activity.
Results: HI-induced motor behavioral deficits showed from 2-week to 8-week after HI injury. In the Rota-rod test, HI group exhibited significantly shorter mean fall latencies as compared to the control group. The d-amphetamine-induced locomotor activity test at the same time point showed reduced locomotor activity in HI group. HI injury resulted in brain structural damages in hippocampus, dorsolateral region of striatum and substantia nigra, and decreases in tyrosine hydroxylase-positive dopaminergic neurons in the substantia nigra. There was no evidence of spontaneous recovery in the substantia nigra at the 8-week after injury.
Conclusion: HI induced brain injury at neonatal period could result in persistent motor behavioral deficits in juvenile rats. Those deficits might be linked with structural damages including nigrostriatal dopaminergic system.

영어초록

Objective: To investigate hypoxia-ischemia induced nigrostriatal system damages and motor behavioral changes in the immature developing rat brain.
Method: For establishment of hypoxia-ischemia (HI) injury, bilateral common carotid artery occlusion was performed permanently with bipolar electrocoagulation in the postnatal day 5 rats. And then rat pups were immediately subjected to hypoxic exposure (8% oxygen) at 37℃ for 1 hour. The control group underwent sham operation and normoxic exposure. Brain injury including striatonigral system was examined. Motor behavioral changes were investigated at 2-, 4-, 6- and 8-week after HI injury using the Rota-rod test and the d-amphetamine-induced locomotor activity.
Results: HI-induced motor behavioral deficits showed from 2-week to 8-week after HI injury. In the Rota-rod test, HI group exhibited significantly shorter mean fall latencies as compared to the control group. The d-amphetamine-induced locomotor activity test at the same time point showed reduced locomotor activity in HI group. HI injury resulted in brain structural damages in hippocampus, dorsolateral region of striatum and substantia nigra, and decreases in tyrosine hydroxylase-positive dopaminergic neurons in the substantia nigra. There was no evidence of spontaneous recovery in the substantia nigra at the 8-week after injury.
Conclusion: HI induced brain injury at neonatal period could result in persistent motor behavioral deficits in juvenile rats. Those deficits might be linked with structural damages including nigrostriatal dopaminergic system.

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