人蔘이 생쥐의 남성 생식세포 GC-1 Spermatogonia의 항산화에 미치는 영향 (Antioxidant Effects of Panax ginseng in Mouse GC-1 Spermatogonia Cells)

서지정보

· 발행기관 : 대한본초학회
· 수록지 정보 : 대한본초학회지 / 24권 / 2호 / 93 ~ 98페이지
· 저자명 : 심경준, 강지웅, 최봉재, 박수연, 장문석, 박성규

초록

Objectives : Previously we reported that the roots of Panax ginseng C.A. Meyer (Araliaceae) increased sperm count and motility, also induced spermatogenesis via cAMP-responsive element modulator(CREM) activation in rat testes. In this study, for the first step of spermatogenesis in germ cell lines, the antioxidant activity of Panax ginseng were examined in mouse GC-1 spermatogonia cells.
Methods : The extract was studied on diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, H2O2-induced cytotoxicity by MTT assay and lipid peroxidation by malondialdehyde (MDA) formation, respectively.
Results : The results showed that the extract scavenged DPPH radical with the IC50 being 0.631 mg/ml. The extract at concentrations of 5, and 10, 50, 100, 250 μg/ml increased GC-1 cell viability significantly(p < 0.05, and p < 0.01). Hydrogen peroxide-induced cytotoxicity (73.8%, p < 0.01) was blocked by the extract at concentrations of 50, and 100, 250, 500 μg/ml significantly (p < 0.05, and p < 0.01). The extract at concentrations of 10, and 50 μg/ml decreased the MDA formation on hydrogen peroxide-induced lipid peroxidation.
Conclusions : In conclusion, the extract of Panax ginseng has potent antioxidant activity and increases the survival rate of GC-1 spg cells against H2O2-induced cytotoxicity.

영어초록

Objectives : Previously we reported that the roots of Panax ginseng C.A. Meyer (Araliaceae) increased sperm count and motility, also induced spermatogenesis via cAMP-responsive element modulator(CREM) activation in rat testes. In this study, for the first step of spermatogenesis in germ cell lines, the antioxidant activity of Panax ginseng were examined in mouse GC-1 spermatogonia cells.
Methods : The extract was studied on diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, H2O2-induced cytotoxicity by MTT assay and lipid peroxidation by malondialdehyde (MDA) formation, respectively.
Results : The results showed that the extract scavenged DPPH radical with the IC50 being 0.631 mg/ml. The extract at concentrations of 5, and 10, 50, 100, 250 μg/ml increased GC-1 cell viability significantly(p < 0.05, and p < 0.01). Hydrogen peroxide-induced cytotoxicity (73.8%, p < 0.01) was blocked by the extract at concentrations of 50, and 100, 250, 500 μg/ml significantly (p < 0.05, and p < 0.01). The extract at concentrations of 10, and 50 μg/ml decreased the MDA formation on hydrogen peroxide-induced lipid peroxidation.
Conclusions : In conclusion, the extract of Panax ginseng has potent antioxidant activity and increases the survival rate of GC-1 spg cells against H2O2-induced cytotoxicity.

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