흰쥐 유양동 폐쇄술 후 시간에 따른 조직학적 변화 (Histologic Serial Changes of Obliterating Materials in the Rat Temporal Dorsal Bullae)

서지정보

· 발행기관 : 대한이비인후과학회
· 수록지 정보 : 대한이비인후-두경부외과학회지 / 52권 / 3호 / 220 ~ 227페이지
· 저자명 : 박정홍, 구태우, 박병건, 장윤석, 강명구

초록

Background and Objectives：Mastoid obliteration surgery is a modern trend for otitis media either with or without cholesteatoma.
The aim of our study is to evaluate histologic changes and effectiveness over time resulting from different obliterating
materials and the existence of mucosa in the temporal dorsal bullae in rats. Materials and Method：Rats were divided into two
groups. One group had the mucosa removed and was treated with trichloroacetic acid (TCA). The other group with mucosa
remaining was untreated. The temporal dorsal bullae of the two groups of rats were obliterated with Mimix® (hydroxyapatite
cement), Regenafil® (demineralized bone matrix), cartilage chip, and bone chip. Three months and again six months after the
implantation, 5 animals in each group were examined. A histological study was performed to evaluate inflammation, new bone
formation, and mucocele formation within the bullae. Results：The group that had Mimix® implanted had a high inflammatory
reaction, low implanted material resorption and cyst formation. The group with Regenafil® implanted had high cyst formation
and more cyst formation with the passage of time. The group with bone chip implanted had high new bone formation, but also
high cyst formation. The group that had cartilage chip implanted had high new bone formation, low implanted material resorption
and low cyst formation. Conclusion：Cartilage chip is the only material that should be used in the air cell with mucosa
remaining. Demineralized bone matrix and bone chip should not be used in the air cell with mucosa remaining. Hydroxyapatite
cement should not be used due to severe inflammation.

영어초록

Background and Objectives：Mastoid obliteration surgery is a modern trend for otitis media either with or without cholesteatoma.
The aim of our study is to evaluate histologic changes and effectiveness over time resulting from different obliterating
materials and the existence of mucosa in the temporal dorsal bullae in rats. Materials and Method：Rats were divided into two
groups. One group had the mucosa removed and was treated with trichloroacetic acid (TCA). The other group with mucosa
remaining was untreated. The temporal dorsal bullae of the two groups of rats were obliterated with Mimix® (hydroxyapatite
cement), Regenafil® (demineralized bone matrix), cartilage chip, and bone chip. Three months and again six months after the
implantation, 5 animals in each group were examined. A histological study was performed to evaluate inflammation, new bone
formation, and mucocele formation within the bullae. Results：The group that had Mimix® implanted had a high inflammatory
reaction, low implanted material resorption and cyst formation. The group with Regenafil® implanted had high cyst formation
and more cyst formation with the passage of time. The group with bone chip implanted had high new bone formation, but also
high cyst formation. The group that had cartilage chip implanted had high new bone formation, low implanted material resorption
and low cyst formation. Conclusion：Cartilage chip is the only material that should be used in the air cell with mucosa
remaining. Demineralized bone matrix and bone chip should not be used in the air cell with mucosa remaining. Hydroxyapatite
cement should not be used due to severe inflammation.

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