저칼륨혈증 흰쥐에서 GTF IIA 유전자의 발현 (Expression and Localization of General TranscriptionFactor IIA Gene in Hypokalemic Rat)

서지정보

· 발행기관 : 대한해부학회
· 수록지 정보 : Anatomy and Cell Biology / 41권 / 1호 / 29 ~ 37페이지
· 저자명 : 이창배, 이용찬, 조혜정, 이송은, 배춘상, 김백윤, 박성식, 안규윤

초록

Potassium balance in chronic hypokalemia is regulated by ion channels, ion transporters, and various
related genes. We isolated general transcription factor IIA (GTF IIA) gene using a DNA chip microassay, a useful method
in cloning genes. Northern analysis and in situ hybridization (ISH) were carried out to analyze the expression and
localization of GTF IIA mRNA in rat in relation to the amount of potassium in the diet. Isoform-specific 32P-labeled
cDNA (Northern analysis) or digoxigenin-labeled cRNA (ISH) probes were used. Northern analysis demonstrated that
GTF IIA mRNA was expressed abundantly in testis; modestly in heart, kidney, lung, adrenal gland, liver, and spleen;
and weakly in brain, distal colon, duodenum, salivary gland, and stomach. In potassium-restricted animals, GTF IIA
expression was decreased in the kidney, adrenal gland, and spleen, but expression was restored to normal levels in
L3w. The expression level in the lung was decreased in L3d and L2w, and increased in L1w and L3w. ISH showed
that mRNA for the GTF IIA gene was detected in the distal convoluted tubule, S3 segment of the proximal tubule, and
cortical collecting duct in the normal group. In potassium-restricted groups, the hybridization signal was detected in
the distal convoluted tubule, S3 segment of the proximal tubule, and entire collecting tubule. The signal intensity of the
outer and inner medullary collecting ducts was higher in the potassium-restricted group than in the normal group but
was decreased in the distal convoluted tubule and S3 segment of the proximal tubule. In the normal group, mRNA of
the GTF IIA gene was detected in the zona glomerulosa cells of the adrenal gland, lymphocytes of the marginal zone,
germinal center of the spleen, and bronchial epithelium and lymphocytes of the lung. mRNA for the GTF IIA gene was
also detected in the cells of the basal portion of the intestinal glands of the distal colon and stomach, and in spermatogonia
and spermatocytes of the seminiferous tubule. These results suggest that expression of GTF IIA differs between
various tissues and that increased expression of the GTF IIA gene in the outer and inner medullary collecting
ducts of the hypokalemic kidney might regulate the ion transporter genes in these segments.

영어초록

Potassium balance in chronic hypokalemia is regulated by ion channels, ion transporters, and various
related genes. We isolated general transcription factor IIA (GTF IIA) gene using a DNA chip microassay, a useful method
in cloning genes. Northern analysis and in situ hybridization (ISH) were carried out to analyze the expression and
localization of GTF IIA mRNA in rat in relation to the amount of potassium in the diet. Isoform-specific 32P-labeled
cDNA (Northern analysis) or digoxigenin-labeled cRNA (ISH) probes were used. Northern analysis demonstrated that
GTF IIA mRNA was expressed abundantly in testis; modestly in heart, kidney, lung, adrenal gland, liver, and spleen;
and weakly in brain, distal colon, duodenum, salivary gland, and stomach. In potassium-restricted animals, GTF IIA
expression was decreased in the kidney, adrenal gland, and spleen, but expression was restored to normal levels in
L3w. The expression level in the lung was decreased in L3d and L2w, and increased in L1w and L3w. ISH showed
that mRNA for the GTF IIA gene was detected in the distal convoluted tubule, S3 segment of the proximal tubule, and
cortical collecting duct in the normal group. In potassium-restricted groups, the hybridization signal was detected in
the distal convoluted tubule, S3 segment of the proximal tubule, and entire collecting tubule. The signal intensity of the
outer and inner medullary collecting ducts was higher in the potassium-restricted group than in the normal group but
was decreased in the distal convoluted tubule and S3 segment of the proximal tubule. In the normal group, mRNA of
the GTF IIA gene was detected in the zona glomerulosa cells of the adrenal gland, lymphocytes of the marginal zone,
germinal center of the spleen, and bronchial epithelium and lymphocytes of the lung. mRNA for the GTF IIA gene was
also detected in the cells of the basal portion of the intestinal glands of the distal colon and stomach, and in spermatogonia
and spermatocytes of the seminiferous tubule. These results suggest that expression of GTF IIA differs between
various tissues and that increased expression of the GTF IIA gene in the outer and inner medullary collecting
ducts of the hypokalemic kidney might regulate the ion transporter genes in these segments.

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