Subrenal Capsul Assay를 이용한 흰쥐와 Zucker 비만쥐에 폐암세포주 이식의 효과 (The Effects of Lung Cancer Cell Line in Spague Dowley Rats and Zucker's Obese Rats Using Subrenal Capsule Assay.)

서지정보

· 발행기관 : 고신대학교(의대) 고신대학교 의과대학 학술지
· 수록지 정보 : 고신대학교 의과대학 학술지 / 20권 / 1호 / 76 ~ 87페이지
· 저자명 : 이동석, 김송명, 김종인, 변정훈

초록

The effects of lung cancer cell line in Spague Dowley rats and Zucker's obese rats using subrenal capsule assay.
Background/Purpose : Lung cancer is well known as one of the high malignant cancer. So far many clinical trials of multimodality treatment consisted of chemotherapy, radiotherapy and surgery were performed, but the results showed quite difference according to the authors and the 5-year survival rate is still dismal. Presently the subrenal capsule assay is regarded as the best method to assess anticancer chemosensitivity and it's importance is increasing gradually. Recently, obesity due to overnutrition and underexercise has been increased. In this study, we evaluated of the tumor growth and the differences of cancer chemosensitivity after human lung cancer cell implantation between Sprague Dowley rats and Zucker obese rats.
Materials and Method : Human lung cancer cells (SW-900G 4) were cultured on the RPMI 1640 media which contains fetal cow serum and fibrin clot which contains 107 cancer cells (2x2x2mm3). Those cultured cells were implanted to a S.D rats were implanted to a S.D rats and a Zucker's obese rates. Group 1 and group 2 were S.D rats and group 3 and group 4 were Zucker obese rats. From the first day to sixth day of implantation, cyclosporin-A (80mg/kg) was given subcutaneously for immunosuppression. Group 1 and 3 were given for normal saline, group 3 and group 4 were given for NIC (Navelbine 10mg/kg, Ifosfamide 30mg/kg, Cis-Platinum 1mg/kg) chemotherapy for 6 days after tumor cell implantation. Body weight and blood glucose level were measured before and after the implantation. Implanted tumor size and it's histological findings were evaluated and the results of each groups were compared.
Result : Because of the NIC chemotherapy, group 2 showed loss of the body weight, but group 4 was not affected by the chemotherapy relatively and the body weight increased slowly by rat's age. The blood glucose level of each groups were within normal range. But its level of group 2 was less than group 1 by 23mg%, but group 3 and 4 have similar glucose level which means those two groups were not affected much by the cancer chemotherapy. The growth of implanted cancer cells in Zucker obese rats was slow than in S.D rats. The cancer cell growth of S.D rats was not inhibited by chemotherapy, but chemotherapy influenced to the cancer cell growth in Zucker obese rats. In the histologic findings, malignant potency was relatively hihgh in group 1 and 2, but low in group 3 and 4. Apoptosis were found in group 2,4. All of groups revealed mild inflammation and fibroblasts were observed in group 1,3.
Conclusion : This study demonstrates that subrenal capsule assay can be used to determine the response of human lung cancer cell to various combinations of chemotherapeutic agents. And the subrenal capsule assay can be done for the chemosensitivity test for lung cancer cells in short time. In the Zucker obese rats, we certified that metabolic state related to obesity influenced to human lung cancer cell growth.

영어초록

The effects of lung cancer cell line in Spague Dowley rats and Zucker's obese rats using subrenal capsule assay.
Background/Purpose : Lung cancer is well known as one of the high malignant cancer. So far many clinical trials of multimodality treatment consisted of chemotherapy, radiotherapy and surgery were performed, but the results showed quite difference according to the authors and the 5-year survival rate is still dismal. Presently the subrenal capsule assay is regarded as the best method to assess anticancer chemosensitivity and it's importance is increasing gradually. Recently, obesity due to overnutrition and underexercise has been increased. In this study, we evaluated of the tumor growth and the differences of cancer chemosensitivity after human lung cancer cell implantation between Sprague Dowley rats and Zucker obese rats.
Materials and Method : Human lung cancer cells (SW-900G 4) were cultured on the RPMI 1640 media which contains fetal cow serum and fibrin clot which contains 107 cancer cells (2x2x2mm3). Those cultured cells were implanted to a S.D rats were implanted to a S.D rats and a Zucker's obese rates. Group 1 and group 2 were S.D rats and group 3 and group 4 were Zucker obese rats. From the first day to sixth day of implantation, cyclosporin-A (80mg/kg) was given subcutaneously for immunosuppression. Group 1 and 3 were given for normal saline, group 3 and group 4 were given for NIC (Navelbine 10mg/kg, Ifosfamide 30mg/kg, Cis-Platinum 1mg/kg) chemotherapy for 6 days after tumor cell implantation. Body weight and blood glucose level were measured before and after the implantation. Implanted tumor size and it's histological findings were evaluated and the results of each groups were compared.
Result : Because of the NIC chemotherapy, group 2 showed loss of the body weight, but group 4 was not affected by the chemotherapy relatively and the body weight increased slowly by rat's age. The blood glucose level of each groups were within normal range. But its level of group 2 was less than group 1 by 23mg%, but group 3 and 4 have similar glucose level which means those two groups were not affected much by the cancer chemotherapy. The growth of implanted cancer cells in Zucker obese rats was slow than in S.D rats. The cancer cell growth of S.D rats was not inhibited by chemotherapy, but chemotherapy influenced to the cancer cell growth in Zucker obese rats. In the histologic findings, malignant potency was relatively hihgh in group 1 and 2, but low in group 3 and 4. Apoptosis were found in group 2,4. All of groups revealed mild inflammation and fibroblasts were observed in group 1,3.
Conclusion : This study demonstrates that subrenal capsule assay can be used to determine the response of human lung cancer cell to various combinations of chemotherapeutic agents. And the subrenal capsule assay can be done for the chemosensitivity test for lung cancer cells in short time. In the Zucker obese rats, we certified that metabolic state related to obesity influenced to human lung cancer cell growth.

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