제초제저항성 유채로부터 교잡근연종으로의 화분을 통한 유전자이동 조사 (Pollen-mediated Gene Flow from Herbicide Resistant Oilseed Rape (Brassica napus L.) to Its Relatives)

서지정보

· 발행기관 : 한국잔디학회
· 수록지 정보 : Weed & Turfgrass Science / 8권 / 2호 / 99 ~ 107페이지
· 저자명 : 이범규, 손수인

초록

The development and use of genetically modified (GM) crops have continuously increased. In particular, canola is the fourth largest GM crop in the world after soy, maize and cotton. Canola (Brassica napus) can hybridize with B. rapa, B. juncea and B. oleraceae, due to its genetic affinity with them, and is known to be hybridizable with various relatives. The commercialization of GM canola requires the establishment of risk assessment techniques. Canola has a relatively long pollen dispersal and can hybridize with wild species; thus, it requires rigorous safety assessment techniques for gene flow. This study investigated gene flow from canola to other Brassicaceae crops in the field area. Herbicide resistant canola was used as the pollen donor and placed at the center, and Chinese cabbage, turnip and leaf mustard were placed in four directions from the canola as pollen recipients. To analyze hybridization, seeds were harvested from the pollen recipient plants, and herbicide resistant seeds were selected by using an imidazolinone herbicide. Then, gene flow was verified through PCR analysis. Chinese cabbage showed a hybridization rate of 0.083% at the close distances of 0-6.1 m, and 0.033% at the distances of 9.15-15.25 m. Turnip showed a hybridization rate of 0.067% at 0-6.1 m from the pollen donor, but no turnip hybrid was found at the distances of 9.15-27.5 m. For leaf mustard, no hybrid was found in this experiment. At greater distances, one turnip hybrid was found at 30.5m and one Chinese cabbage hybrid at 64.05 m. It is expected that the results of this study would be used as basic data for establishing safety management practices for GM canola.

영어초록

The development and use of genetically modified (GM) crops have continuously increased. In particular, canola is the fourth largest GM crop in the world after soy, maize and cotton. Canola (Brassica napus) can hybridize with B. rapa, B. juncea and B. oleraceae, due to its genetic affinity with them, and is known to be hybridizable with various relatives. The commercialization of GM canola requires the establishment of risk assessment techniques. Canola has a relatively long pollen dispersal and can hybridize with wild species; thus, it requires rigorous safety assessment techniques for gene flow. This study investigated gene flow from canola to other Brassicaceae crops in the field area. Herbicide resistant canola was used as the pollen donor and placed at the center, and Chinese cabbage, turnip and leaf mustard were placed in four directions from the canola as pollen recipients. To analyze hybridization, seeds were harvested from the pollen recipient plants, and herbicide resistant seeds were selected by using an imidazolinone herbicide. Then, gene flow was verified through PCR analysis. Chinese cabbage showed a hybridization rate of 0.083% at the close distances of 0-6.1 m, and 0.033% at the distances of 9.15-15.25 m. Turnip showed a hybridization rate of 0.067% at 0-6.1 m from the pollen donor, but no turnip hybrid was found at the distances of 9.15-27.5 m. For leaf mustard, no hybrid was found in this experiment. At greater distances, one turnip hybrid was found at 30.5m and one Chinese cabbage hybrid at 64.05 m. It is expected that the results of this study would be used as basic data for establishing safety management practices for GM canola.

참고자료

· 없음