세포독성과 형태학적 변화 관찰을 통한 5종 에센셜 오일의 안전성 검색 (A Screening of 5 Species Essential Oil's Safety by the Observation of Cytotoxicity and Morphological Change on Cells)

서지정보

· 발행기관 : 한국미용학회
· 수록지 정보 : 한국미용학회지 / 14권 / 3호 / 1006 ~ 1014페이지
· 저자명 : 윤영한, 김경순, 오혜영, 유병수, 김기영

초록

In medical- and esthetic area the aromatherapy has spread widely by using of inhalation, bath and topical massage with essential oil. Thereby the safety, danger of irritation and sensitive reaction and injury of essential oil must be considered for clients and therapists. Therefore, in this study the safety of 5 species essential oils (ginger, cypress, rosemary, lavender and black pepper) was proven by the measurement of MTT-assay and sirius red staining. Liver cell, brain cell and kidney cell were chosen for the cell viability assay and morphological change observations. In this result, ginger and rosemary have shown as 50% of liver cell viability in concentration of 0.0012㎕/㎖ and 0.0014㎕/㎖. Cell lysis and nucleus breaking were shown in concentration of 0.002% cypress and lavender treated liver cell, and 0.001% black pepper and cypress treated brain cell. Ginger, rosemary and lavender were appeared as very low cytotoxicity on brain cell (in concentration of 0.0006㎕/㎖(ginger oil), 0.0011㎕/㎖(rosemary) and 0.00054㎕/㎖(lavender)). And lavender, rosemary and ginger revealed very low toxicity on kidney cell, and cell lysis and nucleus breaking did not observed in concentration of 0.002% lavender and rosemary treated kidney cell. Cypress has shown a low cytotoxicity on liver, brain and kidney cell. In conclusion, 5 essential oils such as ginger, cypress, rosemary, lavender and black pepper may be use carefully in very low dosage for inhalation and the concentrated essential oil should not be directly touched and exposed. Absolute essential oils must be diluted with carrier oil for topical and systematic massage.

영어초록

In medical- and esthetic area the aromatherapy has spread widely by using of inhalation, bath and topical massage with essential oil. Thereby the safety, danger of irritation and sensitive reaction and injury of essential oil must be considered for clients and therapists. Therefore, in this study the safety of 5 species essential oils (ginger, cypress, rosemary, lavender and black pepper) was proven by the measurement of MTT-assay and sirius red staining. Liver cell, brain cell and kidney cell were chosen for the cell viability assay and morphological change observations. In this result, ginger and rosemary have shown as 50% of liver cell viability in concentration of 0.0012㎕/㎖ and 0.0014㎕/㎖. Cell lysis and nucleus breaking were shown in concentration of 0.002% cypress and lavender treated liver cell, and 0.001% black pepper and cypress treated brain cell. Ginger, rosemary and lavender were appeared as very low cytotoxicity on brain cell (in concentration of 0.0006㎕/㎖(ginger oil), 0.0011㎕/㎖(rosemary) and 0.00054㎕/㎖(lavender)). And lavender, rosemary and ginger revealed very low toxicity on kidney cell, and cell lysis and nucleus breaking did not observed in concentration of 0.002% lavender and rosemary treated kidney cell. Cypress has shown a low cytotoxicity on liver, brain and kidney cell. In conclusion, 5 essential oils such as ginger, cypress, rosemary, lavender and black pepper may be use carefully in very low dosage for inhalation and the concentrated essential oil should not be directly touched and exposed. Absolute essential oils must be diluted with carrier oil for topical and systematic massage.

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