황기약침이 전층피부손상 마우스의 치유에 미치는 영향 (Effect of Astragali Radix Pharmacocupuncture for Wound Healing of Full-Thickness Skin Wound in Mouse)

서지정보

· 발행기관 : 대한한방안이비인후피부과학회
· 수록지 정보 : 한방안이비인후피부과학회지 / 31권 / 1호 / 22 ~ 31페이지
· 저자명 : 홍창호, 강경화, 윤화정, 윤현민, 송춘호, 김철홍

초록

Objectives : In this study, we investigated whether Astragali Radix Pharmacocupuncture (ARP) has an effective on the full thickness defect wound healing process of mouse.
Methods : A total of 50 mice (ICR mouse, 7 week-old male) were divided into control group and ARP group.
A single full thickness skin defect was made on the dorsal side of the each mouse using an 8㎜ diameter biopsy punch. Control group were treated with 0.2㎖ saline and ARP group were treat with 0.2㎖ ARP at 8 points around the wound every three days total 4 times during the experimental period. The change in wound size, contraction rate, healing rate, and epithelization rate was measured by digital images taken on days 3, 6, 9, and 13, and evaluated using a digital image analysis program. Tissues were collected for histological analysis, RT-PCR, and Western blot on days 3, 6, 9, and 15.
Results : The results are as follows. ARP group accelerated the rate of wound contraction, wound healing and epithelization compared to the control group. ARP group showed the decrease of inflammatory cells in early inflammatory phase compared to the control group. ARP group upregulated PECAM-1 mRNA and protein expression compared to the control group. ARP group inhibited the scar width and area compared to the control group.
Conclusions: ARP showed positive effects on wound healing through the inhibition of inflammatory reaction and increase of PECAM-1 expression related to the wound healing process.

영어초록

Objectives : In this study, we investigated whether Astragali Radix Pharmacocupuncture (ARP) has an effective on the full thickness defect wound healing process of mouse.
Methods : A total of 50 mice (ICR mouse, 7 week-old male) were divided into control group and ARP group.
A single full thickness skin defect was made on the dorsal side of the each mouse using an 8㎜ diameter biopsy punch. Control group were treated with 0.2㎖ saline and ARP group were treat with 0.2㎖ ARP at 8 points around the wound every three days total 4 times during the experimental period. The change in wound size, contraction rate, healing rate, and epithelization rate was measured by digital images taken on days 3, 6, 9, and 13, and evaluated using a digital image analysis program. Tissues were collected for histological analysis, RT-PCR, and Western blot on days 3, 6, 9, and 15.
Results : The results are as follows. ARP group accelerated the rate of wound contraction, wound healing and epithelization compared to the control group. ARP group showed the decrease of inflammatory cells in early inflammatory phase compared to the control group. ARP group upregulated PECAM-1 mRNA and protein expression compared to the control group. ARP group inhibited the scar width and area compared to the control group.
Conclusions: ARP showed positive effects on wound healing through the inhibition of inflammatory reaction and increase of PECAM-1 expression related to the wound healing process.

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