생쥐 대식세포에서 Moringa oleifera 잎의 MAPK/NF-κB와 Nrf2/HO-1 경로 조절을 통한 항염증 효과 (Anti-Inflammatory Mechanisms of Moringa oleifera through the Regulation of MAPK/NF-κB and Nrf2/HO-1 Pathways in LPS Stimulated RAW 264.7 Cells)

서지정보

· 발행기관 : 대한통합의학회
· 수록지 정보 : 대한통합의학회지 / 13권 / 2호 / 205 ~ 214페이지
· 저자명 : 선민주, 윤현서, 박충무, 윤민경, 이예빈, 박지선

초록

Purpose : Moringa oleifera is widely distributed in Southeast Asia and has been traditionally used for food and medicinal purposes. Despite the many pharmacological effects of M. oleifera against wounds, pain, hepatic disorders, inflammation, and even cancer, the exact mechanisms related to the anti-inflammatory effect have not been evaluated yet. In this study, the anti-inflammatory mechanism of M. oleifera ethanol extract (MOEE) was analyzed by the Griess reaction and western blot analysis in the murine macrophage cell line, RAW 264.7 cells.
Methods : Inflammation was successfully induced by lipopolysaccharide (LPS) stimulation in RAW 264.7 cells and various concentrations of MOEE treatment dose-dependently attenuated nitric oxide (NO) production, inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) expressions in LPS stimulated RAW 264.7 cells. In addition, both inflammatory transcription factors, nuclear factor (NF)-κB and activator protein (AP)-1, were also significantly inhibited their phosphorylation by MOEE treatment. To investigate the effect of MOEE against upstream signaling molecules, the phosphorylated status of mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase (PI3K)/Akt, were analyzed by western blot analysis.
Results : The phosphorylated status of extracellular signal-regulated kinases (ERK), c-Jun NH2-terminal kinase (JNK), p38, and Akt was mitigated by MOEE treatment in a dose-dependent manner. Numerous lines of evidence indicate that intracellular antioxidative systems could play a role as a suppressor of inflammation. MOEE treatment significantly accelerated the expression of heme oxygenase-1 (HO-1), one of the phase II enzymes, and its transcription factor, nuclear factor-erythroid 2-related factor 2 (Nrf2), in LPS stimulated RAW 264.7 cells.
Conclusion : Consequently, these results suggest that MOEE effectively inhibited LPS-initiated inflammatory responses through the regulation of proinflammatory mediators and their signaling molecules as well as the antioxidative mechanism in RAW 264.7 cells, which might be a promising strategy to treat various inflammatory disorders.

영어초록

Purpose : Moringa oleifera is widely distributed in Southeast Asia and has been traditionally used for food and medicinal purposes. Despite the many pharmacological effects of M. oleifera against wounds, pain, hepatic disorders, inflammation, and even cancer, the exact mechanisms related to the anti-inflammatory effect have not been evaluated yet. In this study, the anti-inflammatory mechanism of M. oleifera ethanol extract (MOEE) was analyzed by the Griess reaction and western blot analysis in the murine macrophage cell line, RAW 264.7 cells.
Methods : Inflammation was successfully induced by lipopolysaccharide (LPS) stimulation in RAW 264.7 cells and various concentrations of MOEE treatment dose-dependently attenuated nitric oxide (NO) production, inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) expressions in LPS stimulated RAW 264.7 cells. In addition, both inflammatory transcription factors, nuclear factor (NF)-κB and activator protein (AP)-1, were also significantly inhibited their phosphorylation by MOEE treatment. To investigate the effect of MOEE against upstream signaling molecules, the phosphorylated status of mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase (PI3K)/Akt, were analyzed by western blot analysis.
Results : The phosphorylated status of extracellular signal-regulated kinases (ERK), c-Jun NH2-terminal kinase (JNK), p38, and Akt was mitigated by MOEE treatment in a dose-dependent manner. Numerous lines of evidence indicate that intracellular antioxidative systems could play a role as a suppressor of inflammation. MOEE treatment significantly accelerated the expression of heme oxygenase-1 (HO-1), one of the phase II enzymes, and its transcription factor, nuclear factor-erythroid 2-related factor 2 (Nrf2), in LPS stimulated RAW 264.7 cells.
Conclusion : Consequently, these results suggest that MOEE effectively inhibited LPS-initiated inflammatory responses through the regulation of proinflammatory mediators and their signaling molecules as well as the antioxidative mechanism in RAW 264.7 cells, which might be a promising strategy to treat various inflammatory disorders.

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