표준입도 분산조건에서 나노입자의 THP-1 세포에 대한 독성영향 (Cytotoxicity of Nanoparticles in cultured THP-1 cells under the Standardized Condition of Size Distribution)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 61권 / 2호 / 109 ~ 116페이지
· 저자명 : 이한들, 박주영, 박광식

초록

Effect of nanoparticles on cellular toxicity can be influenced by size and/or size distribution of the testednanoparticles. Therefore, it is necessary to fulfill the requirement of standardized dispersion when toxicity potencies of differenttypes of nanoparticles are compared. In this study, probe-sonicator calibration for delivered acoustic power was performedaccording to the NANoREG protocol for standardized size-distribution of test nanoparticles including silicananoparticles (SiNP), ceria nanopaaticles (CeNP), titania nanoparticles (TiNP), silver nanoparticles (AgNP) and zinc nanoparticles(ZnNP). Based on the protocol, the increase of water temperature by sonication power was monitored and Pac valuewas obtained. When the time of 8 minutes and 25% amplitude calculated from the Pac value was applied to the sonicationof silica nanoparticles, size distribution reached to the effective level, 210 nm < size < 270 nm, Polydispersity index(PDI) < 0.46. The same acoustic power used in the dispersion of silica nanoparticles was applied to the other test nanoparticles,and cytotoxicity test was performed by MTS assay in cultured THP-1 cells treated with nanoparticles for 24 hrs. Asresults, Lethal Concentration 50% (LC50) of silver nanoparticles was 78.44 ppm, which was most toxic among the testnanoparticles. The toxicity compared by the LC50 seemed to be AgNP > SiNP > TiNP > ZnNP > CeNP.

영어초록

Effect of nanoparticles on cellular toxicity can be influenced by size and/or size distribution of the testednanoparticles. Therefore, it is necessary to fulfill the requirement of standardized dispersion when toxicity potencies of differenttypes of nanoparticles are compared. In this study, probe-sonicator calibration for delivered acoustic power was performedaccording to the NANoREG protocol for standardized size-distribution of test nanoparticles including silicananoparticles (SiNP), ceria nanopaaticles (CeNP), titania nanoparticles (TiNP), silver nanoparticles (AgNP) and zinc nanoparticles(ZnNP). Based on the protocol, the increase of water temperature by sonication power was monitored and Pac valuewas obtained. When the time of 8 minutes and 25% amplitude calculated from the Pac value was applied to the sonicationof silica nanoparticles, size distribution reached to the effective level, 210 nm < size < 270 nm, Polydispersity index(PDI) < 0.46. The same acoustic power used in the dispersion of silica nanoparticles was applied to the other test nanoparticles,and cytotoxicity test was performed by MTS assay in cultured THP-1 cells treated with nanoparticles for 24 hrs. Asresults, Lethal Concentration 50% (LC50) of silver nanoparticles was 78.44 ppm, which was most toxic among the testnanoparticles. The toxicity compared by the LC50 seemed to be AgNP > SiNP > TiNP > ZnNP > CeNP.

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