반복적인 오존노출이 마우스에서 기도재형성에 미치는 영향 (Effects of Repeated Ozone Exposure on Airway Remodeling in Mice)

서지정보

· 발행기관 : 대한이비인후과학회
· 수록지 정보 : 대한이비인후-두경부외과학회지 / 51권 / 3호 / 240 ~ 249페이지
· 저자명 : 김용민, 김인중, 김효선, 김아영, 한욱경, 나기상

초록

To investigate whether repeated long-term exposure of ozone has an effect on airway remodeling
and whether ozone exposure has an aggravating effect on airway remodeling during allergen challenge in previously sensitized
mice. Materials and Method：Forty female BALB/c mice were divided into four groups as follows：Group I as the control
group, Group II the ovalbumin aerosol exposure group, Group III the ozone exposure group, and Group IV the ovalbumin
aerosol and ozone exposure group. Twenty-four hours after the last OVA challenge, the concentration of IFN-γ, IL-5, IL-13 and
TGF-β and the count of eosinophil and lymphocyte were measured in nasal lavage fluid (NLF) and bronchoalvelolar lavage
fluid (BALF). Immunohistochemical staining for MMP-9 and TIMP-1 were performed. Trichrome staining was also conducted
to evaluate subepithelial fibrosis. Results：Group II and III exhibited increased IL-5, IL-13, and TGF-β in NLF and BALF,
compared to Group I；Group IV showed more increased pattern for these cytokines, compared to Group II and III. Group II, III,
and IV also showed increased expression of MMP-9 and TIMP-1, compared to Group I in lung；Group II and IV showed more
compared to Group I and III in nasal cavity mucosa. Subepithelial fibrosis was prominent in Group II, III, and IV, compared with
group I. In group IV, subepithelial fibrosis was more increased than Group II and III. Conclusion：These results suggest that
ozone exposure induces remodeling in upper and lower airway and also enhances allergen-mediated airway remodeling in previously
sensitized animals. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:240-9)

영어초록

To investigate whether repeated long-term exposure of ozone has an effect on airway remodeling
and whether ozone exposure has an aggravating effect on airway remodeling during allergen challenge in previously sensitized
mice. Materials and Method：Forty female BALB/c mice were divided into four groups as follows：Group I as the control
group, Group II the ovalbumin aerosol exposure group, Group III the ozone exposure group, and Group IV the ovalbumin
aerosol and ozone exposure group. Twenty-four hours after the last OVA challenge, the concentration of IFN-γ, IL-5, IL-13 and
TGF-β and the count of eosinophil and lymphocyte were measured in nasal lavage fluid (NLF) and bronchoalvelolar lavage
fluid (BALF). Immunohistochemical staining for MMP-9 and TIMP-1 were performed. Trichrome staining was also conducted
to evaluate subepithelial fibrosis. Results：Group II and III exhibited increased IL-5, IL-13, and TGF-β in NLF and BALF,
compared to Group I；Group IV showed more increased pattern for these cytokines, compared to Group II and III. Group II, III,
and IV also showed increased expression of MMP-9 and TIMP-1, compared to Group I in lung；Group II and IV showed more
compared to Group I and III in nasal cavity mucosa. Subepithelial fibrosis was prominent in Group II, III, and IV, compared with
group I. In group IV, subepithelial fibrosis was more increased than Group II and III. Conclusion：These results suggest that
ozone exposure induces remodeling in upper and lower airway and also enhances allergen-mediated airway remodeling in previously
sensitized animals. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:240-9)

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