삼소음 알코올 추출물이 생쥐 면역세포의 시토킨 조절에 미치는 효과 (Effect of Samsoeum on Cytokine Regulation of Mouse T cell)

서지정보

· 발행기관 : 한의병리학회
· 수록지 정보 : 동의생리병리학회지 / 22권 / 4호 / 856 ~ 862페이지
· 저자명 : 류내형, 박성민, 강희, 심범상, 김성훈, 최승훈, 안규석

초록

The purpose of this study was to evaluate the effect of Samsoeum(SSE) on cytokine regulation of mouse T cells. The proliferation of mouse CD4 T cells under the influence of SSE extract was measured. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 in various concentrations of SSE extract, it increased proliferation of CD4 cells by 30% in 50 ㎍/㎖ concentration. The proliferation of CD4 cells increased in 100 ㎍/㎖ and 200 ㎍/㎖. Treatment of CD4+ T cells stimulated by anti-CD3e and anti-CD28 with SSE resulted in reduction of IFN-γ and IL-4 levels. SSE has dose-dependent inhibitory effect on IFN-γ and decreased IL-4 by 70% at 50, 200 ㎍/㎖. Oral administration of SSE resulted in increase of CD8+ T cell population in Balb/c mice by 8%. CD4+ T cells under Th1/Th2 polarizing conditions for 3 days with SSE resulted in decrease of IFN-γ level in Th1 cells by 44% and increase of IL-4 level in Th2 cells by 60%. Experimental results of this study show that SSE induces mouse T-cell to transform into Th2, and increases T-cell population and activation.

영어초록

The purpose of this study was to evaluate the effect of Samsoeum(SSE) on cytokine regulation of mouse T cells. The proliferation of mouse CD4 T cells under the influence of SSE extract was measured. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 in various concentrations of SSE extract, it increased proliferation of CD4 cells by 30% in 50 ㎍/㎖ concentration. The proliferation of CD4 cells increased in 100 ㎍/㎖ and 200 ㎍/㎖. Treatment of CD4+ T cells stimulated by anti-CD3e and anti-CD28 with SSE resulted in reduction of IFN-γ and IL-4 levels. SSE has dose-dependent inhibitory effect on IFN-γ and decreased IL-4 by 70% at 50, 200 ㎍/㎖. Oral administration of SSE resulted in increase of CD8+ T cell population in Balb/c mice by 8%. CD4+ T cells under Th1/Th2 polarizing conditions for 3 days with SSE resulted in decrease of IFN-γ level in Th1 cells by 44% and increase of IL-4 level in Th2 cells by 60%. Experimental results of this study show that SSE induces mouse T-cell to transform into Th2, and increases T-cell population and activation.

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