Pseudomonas aeruginosa KS47에 의한 절삭유의 생물학적 분해 (Biodegradation of Cutting Oil by Pseudomonas aeruginosa KS47)

서지정보

· 발행기관 : 한국미생물학회
· 수록지 정보 : 미생물학회지 / 44권 / 1호 / 22 ~ 28페이지
· 저자명 : 김란희, 이상섭

초록

본실험은생분해가어려운절삭유를단일균주에의해생물학적처리를하는데에목적이있다. 절삭유, 절삭폐유로부터호기균주 81개를분리하여이중절삭유분해능이가장높은균주로, 48시간내에 90.4%를제거한(초기농도 699.1 mg/L) KS47을선별하였다. KS47은형태학적, 생리·화학적, 16S rDNA 염기서열, 그리고지방산분석을 통해Pseudomonas aeruginosa로동정되었다. P. aeruginosa KS47은절삭유를탄소원으로사용하여 성장할수 있었으며, 절삭유 분해시, 최적 분해 조건은 1.5 g/L(wet weight), pH 7.0, 30oC이었다. 최적 조건 하에서 절삭유의 제거능을 본 결과, 1,060 mg/L의 절삭유를 12시간 내에 83.7% 제거함을 확인하였다

영어초록

Cutting oils are emulsionable fluids widely used in metal working processes. Their composition is mineral oil, water, and additives (fatty acids, surfactants, biocides, etc.) generating a toxic waste after a long use. Cutting oilsalso affect colour, taste and odour of water, making it undesirable for domestic and industrial uses. In these days,conventional treatment methods as evaporation, membrane separation or chemical separation have major disadvantages
since they generate a concentrated stream that is more harmful than the original waste. In this study, our purpose is to reduce cutting oils by using biological treatment. Eighty one strains were isolated from cutting waste oil of industrial waste water sludge under aerobic conditions. Among these strains, KS47, which removed 90.4% cutting oil in 48 hr, was obtained by screening test under aerobic conditions (pH 7, 28oC). KS47 was identified as Pseudomonas aeruginosa according to morphological, physiological and biochemical properties, 16S rDNA sequence, and fatty acid analysis. P. aeruginosa KS47 could utilize cutting oil as carbon source. In batch test, we obtained optimal degradation conditions (1.5 g/L cell concentration, pH 7, and temperature 30oC). Under the optimal conditions, 1,060 mg/L cutting oil was removed 83.7% (74.1 mg/L/hr).

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