Acriflavine-Guanosine 복합제(AG60)가 Ehrlich 암세포를 이식한 생쥐 위점막 으뜸세포의 미세구조에 미치는 영향 (Effects of AG60 on Gastric Chief Cells of the Mouse Implanted with Ehrlich Carcinoma Cells)

서지정보

· 발행기관 : 대한해부학회
· 수록지 정보 : Anatomy and Cell Biology / 41권 / 3호 / 193 ~ 204페이지
· 저자명 : 고정식, 왕종원, 박경호, 박대균

초록

This experiment was performed to evaluate the morphological responses of the gastric epithelial cells
and the gastric chief cells of the mouse inoculated with Ehrlich carcinoma cells in the inguinal area following administration
of acriflavine-guanosine composition (AG60). Healthy adult ICR mice were divided into normal and experimental
groups. In the experimental groups, each mouse was inoculated with 1×107 Ehrlich carcinoma cells subcutaneously
in the inguinal area. The day following the 7th injection of saline or AG60, each mouse was injected with
methyl-3H-thymidine through tail vein. Seventy minutes after the thymidine injection, gastric tissues were taken and
fixed in 10% buffered neutral formalin. Deparaffinized sections were coated with autoradiographic emulsion EM-1 and
dried, and then placed in a light-tight box. The number of labeled epithelial cells in the gastric mucosae were observed
and calculated. And for electron microscopic observation, gastric tissues were prefixed with 2.5% glutaraldehyde-1.5%
paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were
stained with uranyl acetate and lead citrate. The size of zymogen granules and mitochondria in the gastric chief cells
were observed and calculated. On the autoradiographic study, number of labeled cells in the area of 3.5 mm width (6
μm thickness) of mouse gastric mucosae of normal control, tumor control and AG60-treated groups were 319.7±
66.46, 343.7±47.72 and 102.3±54.99 respectively. On the electron microscopic study, the size of zymogen granule
in the gastric chief cells of normal control, tumor control and AG60-treated groups were 0.74±0.208 μm, 1.18±0.291
μm and 0.97±0.259 μm, respectively. And the mitochondrial size of the gastric chief cells of normal control, tumor
control and AG60-treated groups were 0.86±0.364 μm, 1.02±0.466 μm and 0.92±0.390 μm, respectively. And in the
AG60 treated group, most chief cells did not show any difference in ultrastructure, except that myelin figures were more
frequently observed, in comparison with that of nornmal control group. From the above results, AG60 may suppress
the DNA synthesis of the gastric epithelial cells, but does not results severe fine structural defect on the gastric chief
cells. These results suggest that AG60 is expected as one of the most effective anticancer drugs.

영어초록

This experiment was performed to evaluate the morphological responses of the gastric epithelial cells
and the gastric chief cells of the mouse inoculated with Ehrlich carcinoma cells in the inguinal area following administration
of acriflavine-guanosine composition (AG60). Healthy adult ICR mice were divided into normal and experimental
groups. In the experimental groups, each mouse was inoculated with 1×107 Ehrlich carcinoma cells subcutaneously
in the inguinal area. The day following the 7th injection of saline or AG60, each mouse was injected with
methyl-3H-thymidine through tail vein. Seventy minutes after the thymidine injection, gastric tissues were taken and
fixed in 10% buffered neutral formalin. Deparaffinized sections were coated with autoradiographic emulsion EM-1 and
dried, and then placed in a light-tight box. The number of labeled epithelial cells in the gastric mucosae were observed
and calculated. And for electron microscopic observation, gastric tissues were prefixed with 2.5% glutaraldehyde-1.5%
paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were
stained with uranyl acetate and lead citrate. The size of zymogen granules and mitochondria in the gastric chief cells
were observed and calculated. On the autoradiographic study, number of labeled cells in the area of 3.5 mm width (6
μm thickness) of mouse gastric mucosae of normal control, tumor control and AG60-treated groups were 319.7±
66.46, 343.7±47.72 and 102.3±54.99 respectively. On the electron microscopic study, the size of zymogen granule
in the gastric chief cells of normal control, tumor control and AG60-treated groups were 0.74±0.208 μm, 1.18±0.291
μm and 0.97±0.259 μm, respectively. And the mitochondrial size of the gastric chief cells of normal control, tumor
control and AG60-treated groups were 0.86±0.364 μm, 1.02±0.466 μm and 0.92±0.390 μm, respectively. And in the
AG60 treated group, most chief cells did not show any difference in ultrastructure, except that myelin figures were more
frequently observed, in comparison with that of nornmal control group. From the above results, AG60 may suppress
the DNA synthesis of the gastric epithelial cells, but does not results severe fine structural defect on the gastric chief
cells. These results suggest that AG60 is expected as one of the most effective anticancer drugs.

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