越婢湯이 高脂肪食餌로 유도된 비만 생쥐에 미치는 영향 (Anti-obesity Effects of Wolbi-tang(越婢湯) on the Obese-mice Induced by High-fat Diet)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 21권 / 2호 / 31 ~ 48페이지
· 저자명 : 박지현, 홍서영

초록

Objectives :In order to investigate the anti-obesity effects of Wolbi-tang(here in after referred to WBT) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high-fat diet.
Methods :C57BL/6 mice were divided into 5 groups(normal, only high-fat diet, high-fat diet with Reductil, high-fat diet with WBT 400, 200 mg/kg extract) and fed for 5 weeks. And observed body weight change, total cholesterol, low density lipoprotein cholesterol(LDL-cholesterol), high density lipoprotein cholesterol (HDL-cholesterol), triglyceride, glucose, leptin change, alanine transaminase(ALT), aspartate transaminase(AST), serum creatinine, the expression of β3-adrenergic receptor(β3AR), leptin, uncoupling protein(UCP2) gene in 3T3-L1 adipocyte, 3T3-L1 adipocyte proliferation, histological analysis of adipose tissue and liver tissue.
Results :1. Refer to cell cytotoxicity, viability of human fibroblast cells(hFCs) showed not significant changes.
2. The amount of ALT, AST was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. The amount of creatinine showed not significant changes.
3. Body weight was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups.
4. The amount of total cholesterol and triglyceride was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. LDL-cholesterol was decreased and HDL-cholesterol was increased significantly in WBT 400 mg/kg group.
5. The amount of glucose was decreased significantly in WBT 400 mg/kg group.
6. The amount of serum leptin was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups.
7. The revelation of β3AR in 3T3-L1 adipocyte was increased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of leptin was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of UCP2 was decreased significantly in WBT 100 μg/ml group.
8. 3T3-L1 adipocyte proliferation was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The size of adipocyte was decreased relative to the control group in WBT 400 mg/kg group.
9. The adipose vacuoles in liver tissue was decreased relative to the control group.
Conclusions :These results suggested that WBT has inhibitory effects of obesity. WBT might be applicated on treatment of obesity and metabolic syndrome. Further studies analysing its effects were needed.

영어초록

Objectives :In order to investigate the anti-obesity effects of Wolbi-tang(here in after referred to WBT) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high-fat diet.
Methods :C57BL/6 mice were divided into 5 groups(normal, only high-fat diet, high-fat diet with Reductil, high-fat diet with WBT 400, 200 mg/kg extract) and fed for 5 weeks. And observed body weight change, total cholesterol, low density lipoprotein cholesterol(LDL-cholesterol), high density lipoprotein cholesterol (HDL-cholesterol), triglyceride, glucose, leptin change, alanine transaminase(ALT), aspartate transaminase(AST), serum creatinine, the expression of β3-adrenergic receptor(β3AR), leptin, uncoupling protein(UCP2) gene in 3T3-L1 adipocyte, 3T3-L1 adipocyte proliferation, histological analysis of adipose tissue and liver tissue.
Results :1. Refer to cell cytotoxicity, viability of human fibroblast cells(hFCs) showed not significant changes.
2. The amount of ALT, AST was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. The amount of creatinine showed not significant changes.
3. Body weight was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups.
4. The amount of total cholesterol and triglyceride was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. LDL-cholesterol was decreased and HDL-cholesterol was increased significantly in WBT 400 mg/kg group.
5. The amount of glucose was decreased significantly in WBT 400 mg/kg group.
6. The amount of serum leptin was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups.
7. The revelation of β3AR in 3T3-L1 adipocyte was increased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of leptin was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of UCP2 was decreased significantly in WBT 100 μg/ml group.
8. 3T3-L1 adipocyte proliferation was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The size of adipocyte was decreased relative to the control group in WBT 400 mg/kg group.
9. The adipose vacuoles in liver tissue was decreased relative to the control group.
Conclusions :These results suggested that WBT has inhibitory effects of obesity. WBT might be applicated on treatment of obesity and metabolic syndrome. Further studies analysing its effects were needed.

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