사람 호흡기 상피세포에서 Ginsenoside RB1이 TGF-β1로 유도된 MUC4/5AC 점액 유전자의 발현과 상피-중간엽 전이에 미치는 효과 (Ginsenoside Rb1 Attenuates TGF-β1-Induced MUC4/5AC Expression and Epithelial-Mesenchymal Transition in Human Airway Epithelial Cells)

서지정보

· 발행기관 : 대한이비인후과학회
· 수록지 정보 : 대한이비인후-두경부외과학회지 / 64권 / 4호 / 232 ~ 239페이지
· 저자명 : 최태영, 김준희, 조수연, 이상재, 나형균, 최윤석, 송시연, 김용대, 배창훈

초록

Background and Objectives Ginsenoside Rb1 is the main metabolite of Panax ginseng. Itis known to have many beneficial properties including anti-inflammatory, antitumoral and antioxidanteffects. However, the therapeutic effects of ginenoside Rb1 on inflammatory airwaydiseases have not been elucidated. Therefore, we investigated the effects of ginsenoside Rb1 onthe TGF-β1-induced mucin gene expression and epithelial-mesenchymal transition (EMT) inhuman airway epithelial cells.
Materials and Method We evaluated the effects of ginsenoside Rb1 on the changes of MUC4,MUC5AC, occludin, claudin 4, claudin 18, neural (N)-cadherin, and epithelial (E)-cadherin expressionby TGF-β1 in NCI-H292 cells using reverse, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot.
Results TGF-β1 significantly increased MUC4/5AC expression. Rb1 inhibited TGF-β1- inducedMUC4/5AC expression. In addition, TGF-β1 significantly attenuated occludin, claudin 18,and E-cadherin expressions but induced claudin 4 and N-cadherin expressions. On the otherhand, Rb1 reversed changes in the TGF-β1- mediated expressions of cell junction molecules.
Conclusion These results suggest that ginsenoside Rb1 attenuates TGF-β1-induced MUC4/5AC expressions and EMT in the human airway epithelial cells. These findings are importantdata demonstrating the potential of ginsenoside Rb1 as a therapeutic agent for inflammatoryairway diseases

영어초록

Background and Objectives Ginsenoside Rb1 is the main metabolite of Panax ginseng. Itis known to have many beneficial properties including anti-inflammatory, antitumoral and antioxidanteffects. However, the therapeutic effects of ginenoside Rb1 on inflammatory airwaydiseases have not been elucidated. Therefore, we investigated the effects of ginsenoside Rb1 onthe TGF-β1-induced mucin gene expression and epithelial-mesenchymal transition (EMT) inhuman airway epithelial cells.
Materials and Method We evaluated the effects of ginsenoside Rb1 on the changes of MUC4,MUC5AC, occludin, claudin 4, claudin 18, neural (N)-cadherin, and epithelial (E)-cadherin expressionby TGF-β1 in NCI-H292 cells using reverse, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot.
Results TGF-β1 significantly increased MUC4/5AC expression. Rb1 inhibited TGF-β1- inducedMUC4/5AC expression. In addition, TGF-β1 significantly attenuated occludin, claudin 18,and E-cadherin expressions but induced claudin 4 and N-cadherin expressions. On the otherhand, Rb1 reversed changes in the TGF-β1- mediated expressions of cell junction molecules.
Conclusion These results suggest that ginsenoside Rb1 attenuates TGF-β1-induced MUC4/5AC expressions and EMT in the human airway epithelial cells. These findings are importantdata demonstrating the potential of ginsenoside Rb1 as a therapeutic agent for inflammatoryairway diseases

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