理中湯이 IgE가 과잉생성되고 피부염이 유발된 NC/Nga생쥐에서 IFN-γ 반응에 의한 Th2 세포분화 억제에 미치는 영향 (The Effect of Li Zhong Tang on the Suppression of Th2 Differentiation by IFN-γ Response in IgE Hyperproduction and Atopic Dermatitis-like Skin Lesions Induced NC/Nga Mouse)

서지정보

· 발행기관 : 대한한방소아과학회
· 수록지 정보 : 대한한방소아과학회지 / 23권 / 1호 / 1 ~ 22페이지
· 저자명 : 서희연, 김윤희, 한재경

초록

Objectives
The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse.
Methods
First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of IFN-γ, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo , after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, IFN-γ level and observed the changes of activated cell.
Results
In vitro, LZH-T decreased the levels of CD4+/IFN-γ+ and increased the levels of CD4+/GATA3+.
In vivo, serum IgE levels were decreased and IFN-γ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group.
Conclusions
This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

영어초록

Objectives
The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse.
Methods
First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of IFN-γ, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo , after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, IFN-γ level and observed the changes of activated cell.
Results
In vitro, LZH-T decreased the levels of CD4+/IFN-γ+ and increased the levels of CD4+/GATA3+.
In vivo, serum IgE levels were decreased and IFN-γ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group.
Conclusions
This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

참고자료

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