NC/Nga 생쥐에서 加味淸心蓮子湯의 GATA-3 조절에 의한항아토피 피부염 효과 (Anti-dermatitis Effects of KamiCheongsimyeonjatang on GATA-3 Regulation in NC/Nga Mouse)

서지정보

· 발행기관 : 대한한방소아과학회
· 수록지 정보 : 대한한방소아과학회지 / 23권 / 2호 / 29 ~ 50페이지
· 저자명 : 박슬기, 김윤희, 한재경

초록

Objectives
The purpose of this study is to investigate effectiveness of KamiCheongsimyeonjatang (KCSYJT) medicines to suppress atopic dermatitis in mouse model experimentally.
Methods
First, in vitro, we isolated B cells from 18 weeks of atopic dermatitis-like skin NC/Nga mouse. Then we analyzed FACS(Fluorescence Activated Cell Sorter)by intracellular staining of IFN-γ, GATA-3+ and analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of KCSYJT to atopic dermatitis NC/Nga mouse at 12 weeks of age, we analyzed serum IgE and the change of activated cell in PBMCs(Peripheral Blood Mononuclear Cells).
Results
In vitro, KCSYJT medicines supressed IL-1β, IL-6, TNF-α, and TGF-β mRNA and increased IL-10 mRNA in B cells. Also, KCSYJT medicines decreased the levels of GATA-3⁺CD4⁺ and increased the levels of IFN-γ⁺CD4⁺ in CD4⁺ T cell.
In vivo, serum IgE levels decreased in KCSYJT group than control group and In PBMCs, the activated cell percentage of granulocytes, CD3+, CD3+/CD4+, B220+/CD23+, and CCR3+ decreased and CD19+, CD3+/CD8+ increased in KCSYJT group than control group.
Conclusions
This study demonstrates immunological activity of KCSYJT on atopic dermatitis-like model mice.

영어초록

Objectives
The purpose of this study is to investigate effectiveness of KamiCheongsimyeonjatang (KCSYJT) medicines to suppress atopic dermatitis in mouse model experimentally.
Methods
First, in vitro, we isolated B cells from 18 weeks of atopic dermatitis-like skin NC/Nga mouse. Then we analyzed FACS(Fluorescence Activated Cell Sorter)by intracellular staining of IFN-γ, GATA-3+ and analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of KCSYJT to atopic dermatitis NC/Nga mouse at 12 weeks of age, we analyzed serum IgE and the change of activated cell in PBMCs(Peripheral Blood Mononuclear Cells).
Results
In vitro, KCSYJT medicines supressed IL-1β, IL-6, TNF-α, and TGF-β mRNA and increased IL-10 mRNA in B cells. Also, KCSYJT medicines decreased the levels of GATA-3⁺CD4⁺ and increased the levels of IFN-γ⁺CD4⁺ in CD4⁺ T cell.
In vivo, serum IgE levels decreased in KCSYJT group than control group and In PBMCs, the activated cell percentage of granulocytes, CD3+, CD3+/CD4+, B220+/CD23+, and CCR3+ decreased and CD19+, CD3+/CD8+ increased in KCSYJT group than control group.
Conclusions
This study demonstrates immunological activity of KCSYJT on atopic dermatitis-like model mice.

참고자료

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