凉血除濕湯이 DNCB로 유도된 NC/Nga mice의 아토피 피부염에 미치는 영향 (Effect of Yanghyeoljeseuptang (YHJST) Composition on Atopic Dermatitis NC/Nga Mice Induced by DNCB(dinitrochlorobenzene))

서지정보

· 발행기관 : 대한한방소아과학회
· 수록지 정보 : 대한한방소아과학회지 / 23권 / 3호 / 263 ~ 291페이지
· 저자명 : 박두병, 김윤희, 한재경

초록

Objectives
The purpose of this study is to investigate the effect of YHJST on atopic dermatitis in an experiment using an NC/Nga mice induced by DNCB, which has histological and clinical similarities to the condition in humans.
Methods
To investigate the effect of YHJST on atopic dermatitis(AD), we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs) and performed skin histology in ears and dorsal skin of NC/Nga ato-mouse.
Results
YHJST medicines decreased Serum level of IgE, IL-6, TNF-α. Also total number of CD69+, CD3+ in PBMCs, absolute cell number of CCR3+CD3+, CD11b+Gr-1+ in Dorsal skin tissue, Serum IgG1, IgM, IgG2a and IgG2b decreased significantly. Furthermore YHJST is extremely effective to histological symptoms; dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration and suppressed histologic infiltration of CD4+& CCR3+ in ear and dorsal skin lesions significantly. YHJST decreased gene-expression of IL-6, TNF-α, CCR3, Eotaxin mRNA than that of control group.
Conclusions
YHJST on atopic dermatitis to atopic dermatitis NC/Nga mouse induced DNCB was incredibly effective.

영어초록

Objectives
The purpose of this study is to investigate the effect of YHJST on atopic dermatitis in an experiment using an NC/Nga mice induced by DNCB, which has histological and clinical similarities to the condition in humans.
Methods
To investigate the effect of YHJST on atopic dermatitis(AD), we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs) and performed skin histology in ears and dorsal skin of NC/Nga ato-mouse.
Results
YHJST medicines decreased Serum level of IgE, IL-6, TNF-α. Also total number of CD69+, CD3+ in PBMCs, absolute cell number of CCR3+CD3+, CD11b+Gr-1+ in Dorsal skin tissue, Serum IgG1, IgM, IgG2a and IgG2b decreased significantly. Furthermore YHJST is extremely effective to histological symptoms; dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration and suppressed histologic infiltration of CD4+& CCR3+ in ear and dorsal skin lesions significantly. YHJST decreased gene-expression of IL-6, TNF-α, CCR3, Eotaxin mRNA than that of control group.
Conclusions
YHJST on atopic dermatitis to atopic dermatitis NC/Nga mouse induced DNCB was incredibly effective.

참고자료

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