가토의 하악관절에 Collagenase 주입을 통한 관절염 유발 모델에 관한 연구 (Arthritis on Temporomandibular Joint in Rabbit by Collagenase Injection)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 32권 / 6호 / 497 ~ 503페이지
· 저자명 : 송동석, 김기현, 이재열, 정유진, 안상욱, 송진우, 김철훈, 신상훈, 정인교

초록

Purpose: The purpose of this study is to induce artificial arthritis on rabbit TMJ by injecting collagenase.


An experimental animal model of arthritis induced by surgical method or intra-articular injection of chemical agent like LDH, papain, ketorolac. Surgical method is complex and needs a long time in inducing arthritis. Intra-articular injection of chemical agent like LDH, papain, ketorolac is simple. But chemical agent like LDH, papain, ketololac needs multiple injections to induce arthritis and mechanism inducing arthritis was known. Collagenase destroys helical domain of type II collagen in extracellular matrix produced by chondrocyte and then induces arthritis. we injected collagenase(0.5, 1.0, 2.0mg) into the temporomandibular joint of rabbit. In the control group saline was intra-articularly injected. The condylar cartilage, disk and synovia were histologically examined at 1,2,4,6 weeks after the initiation of collagenase injections. Four weeks after injection of 2.0mg collagenase, we could see histologic change like arthritis.
Conclusion: In our study, we produce arthritis on temporomandibular joint of rabbit by using injection of collagenase in temporomandibular joint of rabbit. And this experimental osteoarthritis is a useful animal model.

영어초록

Purpose: The purpose of this study is to induce artificial arthritis on rabbit TMJ by injecting collagenase.


An experimental animal model of arthritis induced by surgical method or intra-articular injection of chemical agent like LDH, papain, ketorolac. Surgical method is complex and needs a long time in inducing arthritis. Intra-articular injection of chemical agent like LDH, papain, ketorolac is simple. But chemical agent like LDH, papain, ketololac needs multiple injections to induce arthritis and mechanism inducing arthritis was known. Collagenase destroys helical domain of type II collagen in extracellular matrix produced by chondrocyte and then induces arthritis. we injected collagenase(0.5, 1.0, 2.0mg) into the temporomandibular joint of rabbit. In the control group saline was intra-articularly injected. The condylar cartilage, disk and synovia were histologically examined at 1,2,4,6 weeks after the initiation of collagenase injections. Four weeks after injection of 2.0mg collagenase, we could see histologic change like arthritis.
Conclusion: In our study, we produce arthritis on temporomandibular joint of rabbit by using injection of collagenase in temporomandibular joint of rabbit. And this experimental osteoarthritis is a useful animal model.

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