加味消風散이 염증 및 아토피피부염 동물병태에 미치는 영향 (Effect of Gami-sopungsan on Inflammation and DNCB-induced Dermatitis in NC/Nga in Mice)

서지정보

· 발행기관 : 한의병리학회
· 수록지 정보 : 동의생리병리학회지 / 28권 / 2호 / 146 ~ 153페이지
· 저자명 : 이해진, 심부용, 박지원, 김동희

초록

Gami-Sopungsan (GS) is one of the traditional korean remedy. We investigated the anti-inflammation and anti-atopic dermatitis (AD) effect of GS. No cytotoxicity of GS was observed in the range of 1~100 ㎍/㎖ on Raw 264.7 cells. The Inflammatory response of Raw 264.7 cells were induced by lipopolysaccharide (LPS), followed by GS treatment at indicated concentrations (0, 1, 10 and 100 ㎍/㎖). At 100 ㎍/㎖ concentration, GS showed inhibitory effect on LPS-induced nitric oxide production by 20%. Production of IL-1β, IL-6 and TNF-α was decreased by approximately 56%, 36% and 79%, respectively upon GS treatment at 100 ㎍/㎖. 200 ㎎/㎏ of GS was orally administered to NC/Nga mice, where AD was induced by 1-chloro 2,4-dinitrobenzene. There were no significant difference between GS treated group and the control group on body weight and food intake changes during growth. The back skin of GS group showed decrease in erythema, pruritus, dry skin, edema, excoriation, erosion and lichenification level through naked eye observations. In addition, leukocyte infiltration and the thickness of epidermis were significantly decreased in the skin tissues (back and ear). The serum IgE levels were decreased by 28.8% in the GS treated group. The GS treated group showed remarkable inhibition of IL-4 (83%), IL-5 (95%), IL-6 (62%) and TNF-α (84%) in serum, indicating that GS has similar or higher efficacy than those of the dexamethasone treated group. From the results above, we conclude that GS has significant anti-inflammation and anti-AD effects on Raw 264.7 cells and NC/Nga mice. The results should provide fundamental and valuable data for the research on natural products being developed against atopic dermatitis.

영어초록

Gami-Sopungsan (GS) is one of the traditional korean remedy. We investigated the anti-inflammation and anti-atopic dermatitis (AD) effect of GS. No cytotoxicity of GS was observed in the range of 1~100 ㎍/㎖ on Raw 264.7 cells. The Inflammatory response of Raw 264.7 cells were induced by lipopolysaccharide (LPS), followed by GS treatment at indicated concentrations (0, 1, 10 and 100 ㎍/㎖). At 100 ㎍/㎖ concentration, GS showed inhibitory effect on LPS-induced nitric oxide production by 20%. Production of IL-1β, IL-6 and TNF-α was decreased by approximately 56%, 36% and 79%, respectively upon GS treatment at 100 ㎍/㎖. 200 ㎎/㎏ of GS was orally administered to NC/Nga mice, where AD was induced by 1-chloro 2,4-dinitrobenzene. There were no significant difference between GS treated group and the control group on body weight and food intake changes during growth. The back skin of GS group showed decrease in erythema, pruritus, dry skin, edema, excoriation, erosion and lichenification level through naked eye observations. In addition, leukocyte infiltration and the thickness of epidermis were significantly decreased in the skin tissues (back and ear). The serum IgE levels were decreased by 28.8% in the GS treated group. The GS treated group showed remarkable inhibition of IL-4 (83%), IL-5 (95%), IL-6 (62%) and TNF-α (84%) in serum, indicating that GS has similar or higher efficacy than those of the dexamethasone treated group. From the results above, we conclude that GS has significant anti-inflammation and anti-AD effects on Raw 264.7 cells and NC/Nga mice. The results should provide fundamental and valuable data for the research on natural products being developed against atopic dermatitis.

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