방풍통성산이 흰쥐의 Adjuvant 유발 관절염에 미치는 영향 (Effects of Bangpungtongseong-san(Fangfengtongsheng-san) on the Adjuvant-induced Arthritis in Rats)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 22권 / 2호 / 91 ~ 103페이지
· 저자명 : 엄관준, 김지영, 김순중, 서일복, 정수현

초록

Objectives :This study was carried out to investigate the effects of Bangpungtongseong-san(Fangfengtongsheng-san) treatment on the change of the adjuvant-induced arthritis in rats.
Methods :Arthritis was induced by injection of complete Freund's adjuvant(CFA, 0.1 ml) into the tail base of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. The control group was taken distilled water and the treated group, extracts of Bangpungtongseong-san(Fangfengtongsheng-san) by orally for 15 days.
At the end of the experiment(28 days after CFA injection), gross and histopathological examinations on the articular structures of ankle joints were performed. The total number of white blood cells was measured by the ADVIA(automated blood cell counter, Bayer Co., Ireland). And also, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) contents in paw exudate of rats were measured by enzyme-linked immunosorbent assay(ELISA) method.
Results :1. Body weight(g) gain rate(%) of the treated group was increased significantly compared with control group at the 15th day after injection.
2. The thickness of ankle joints of the treated group was decreased significantly compared with the control group.
3. Histopathologically, finding of fibrous ankylosis of the treated group was decreased compared with the control group.
4. The total number of white blood cells in the treated group was decreased significantly compared with the control group.
5. TNF-α content and IL-1β content in paw exudate of the treated group were decreased significantly compared with the control group.
Conclusions :On the basis of these results, we suggest that Bangpungtongseong-san(Fangfengtongsheng-san) has inhibiting effects on the progression of arthritis in CFA-induced arthritis model.

영어초록

Objectives :This study was carried out to investigate the effects of Bangpungtongseong-san(Fangfengtongsheng-san) treatment on the change of the adjuvant-induced arthritis in rats.
Methods :Arthritis was induced by injection of complete Freund's adjuvant(CFA, 0.1 ml) into the tail base of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. The control group was taken distilled water and the treated group, extracts of Bangpungtongseong-san(Fangfengtongsheng-san) by orally for 15 days.
At the end of the experiment(28 days after CFA injection), gross and histopathological examinations on the articular structures of ankle joints were performed. The total number of white blood cells was measured by the ADVIA(automated blood cell counter, Bayer Co., Ireland). And also, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) contents in paw exudate of rats were measured by enzyme-linked immunosorbent assay(ELISA) method.
Results :1. Body weight(g) gain rate(%) of the treated group was increased significantly compared with control group at the 15th day after injection.
2. The thickness of ankle joints of the treated group was decreased significantly compared with the control group.
3. Histopathologically, finding of fibrous ankylosis of the treated group was decreased compared with the control group.
4. The total number of white blood cells in the treated group was decreased significantly compared with the control group.
5. TNF-α content and IL-1β content in paw exudate of the treated group were decreased significantly compared with the control group.
Conclusions :On the basis of these results, we suggest that Bangpungtongseong-san(Fangfengtongsheng-san) has inhibiting effects on the progression of arthritis in CFA-induced arthritis model.

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