Thermoresistant properties of bacterioferritin comigratory protein against high temperature stress in Schizosaccharomyces pombe

서지정보

· 발행기관 : 한국미생물학회
· 수록지 정보 : 미생물학회지 / 52권 / 4호 / 398 ~ 405페이지
· 저자명 : 류인왕, 이수희, 임혜원, 안기섭, 박광학, 사재훈, 정경진, 임창진, 김경훈

초록

The Schizosaccharomyces pombe structural gene encoding bacterioferritin comigratory protein (BCP) was previously cloned using the shuttle vector pRS316 to generate the BCP-overexpressing plasmid pBCP10. The present work aimed to evaluate the thermoresistant properties of BCP against high temperature stress using the plasmid pBCP10. When the S. pombe cells were grown to the early exponential phase and shifted from 30°C to 37°C or 42°C, the S. pombe cells harboring pBCP10 grew significantly more at both 37°C and 42°C than the vector control cells. After 6 h of the shifting to higher incubation temperatures, they contained the lower reactive oxygen species (ROS) and nitrite content, an index of nitric oxide (NO), than the vector control cells. After the temperature shifts, total glutathione (GSH) content and total superoxide dismutase (SOD) activities were much higher in the S. pombe cells harboring pBCP10 than in the corresponding vector control cells. Taken together, the S. pombe BCP plays a thermoresistant role which might be based upon its ability both to down-regulate ROS and NO levels and to up-regulate antioxidant components, such as total GSH and SOD, and subsequently to maintain thermal stability.

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