Cell-type Specific Activation of MAPKs in the Progression of Gastric Ulcer in Rats

서지정보

· 발행기관 : 한국임상수의학회
· 수록지 정보 : 한국임상수의학회지 / 30권 / 5호 / 339 ~ 345페이지
· 저자명 : 유리, 권영삼, 오태호, 김태환, 박상준

초록

Mitogen-activated protein kinases (MAPKs) are a family of central signaling molecules that respond to
numerous stimuli and are known to participate in processes of cell survival and death. However, it is not clear on
data for cell-type specific activation of MAPKs in the progression of gastric ulcer. In the present study, we assessed
how MAPKs localized at various cell types during the progression of gastric ulcer induced by ibuprofen. Gastric ulcer
was induced by the repeated treatment of 200 mg/kg ibuprofen with 8 hrs interval in a day. Animals were sacrificed
at 24 hrs, 48 hrs, and 72 hrs after oral treatment of ibuprofen and gastric tissues were subjected to immunohistochemical
and immunoblotting evaluation. Immunoreactivity of phospho-extracellular signal-regulated kinase (p-ERK) was mainly
expressed at the proliferating zone of gastric mucosa in control rats. But, these signals for p-ERK were highly shifted
from cells of proliferating zone to parietal cells of the basal regions 24 hrs after treatment of ibuprofen. p-ERK signal
was strongly expressed in epithelial cells adjacent to ulcer margin and new capillary and infiltrated inflammatory cells
within granulation tissue of the ulcer base above 48 hrs after treatment of ibuprofen. While, phospho-c-Jun NH2 terminal
kinase (p-JNK) was mainly localized to the nuclei of the surface epithelial cells and the glandular epithelial cells in
early gastric injury. Also, p-JNK was often observed as a scattered pattern in different regions of gastric mucosa with
early gastric injury. Gradually, signal of p-JNK was strongly stained in infiltrated inflammatory cells and fibroblasts
within severe ulcer base. Phospho-p38 (p-p38) MAPK was observed as scattered pattern within connective tissues of
gastric mucosa. Especially, p-p38 MAPK showed strong signal in infiltrated macrophages within ulcer base. These
results show that each MAPK has a specific role in various cell types during the progression of gastric ulcer.

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