NF-κB 활성 조절을 통한 Lactobacillus johnsonii 파쇄액의 항염 효과 (Anti-inflammatory Effects of Lactobacillus johnsonii Lysate via Regulation of NF-κB Activity)

서지정보

· 발행기관 : 사단법인 대한화장품학회
· 수록지 정보 : 대한화장품학회지 / 49권 / 3호 / 285 ~ 290페이지
· 저자명 : 차화준

초록

본 연구에서는 김치유래의 Lactobacillus johnsonii의 anti-inflammation 효능을 확인하였다. Rat 유래대식세포인 Raw 264.7세포에 Lactobacillus johnsonii 파쇄물을 처리하여 염증유발 marker인 TNFα와 IL1β의발현량을 확인한 결과 250 μg/mL 추출물을 처리시 1 μg/mL LPS를 처리한 대조군 대비 각각 40.55%와 34.66%의TNFα와 IL1β의 발현량 감소를 확인하였다. 또한 LPS에 의한 cytokine 발현의 핵심 전사인자인 NF-κB의 전사활성을 확인한결과 1 μg/mL LPS를 처리한 대조군 대비 NF-κB의 전사활성은 40.76% 억제됨을 확인하였다. 때문에본 연구결과를 통해 Lactobacillus johnsonii 파쇄물은 LPS에 의한 cytokine의 발현을 방지 및 NF-κB전사활성조절을 통해 항염 또는 피부진정 기능성 소재로써 가능성을 확인하였다.

영어초록

In this study, the anti-inflammation efficacy of Lactobacillus johnsonii derived from Kimchi was investigated.
Raw 264.7 cells, which are rat-derived macrophages, were treated with Lactobacillus johnsonii lysate to confirm the expression level of TNFα and IL1β, which are inflammatory markers, and when treating 250 µg/mL extract, the exp ression level of TNFα and IL1β decreased by 40.55% and 34.66% compared to the control group treated with 1 µg/mL LP, respectively. In addition, as a result of confirming the transcriptional activity of NF-κB, a key transcription factor in cytokine expression by LPS, it was confirmed that the transcriptional activity of NF-κB was 40.76% inhibited compared to the control group treated with 1 µg/mL LPS. Therefore, the results of this study confirmed that Lactobacillus johnsonii lysate is likely to be an anti-inflammatory or skin-soothing functional material by preventing the expression of cytokine by LPS and controlling NF-κB transcriptional activity.

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