인 췌장암세포에 대한 Insulin 및 Insulin-like growth factor (IGF-1)의 성장촉진효과 (Insulin and IGF-1 Stimulate the Growth of Human Pancreatic Cancer Cells in vitro)

서지정보

· 발행기관 : 고신대학교(의대) 고신대학교 의과대학 학술지
· 수록지 정보 : 고신대학교 의과대학 학술지 / 11권 / 1호 / 19 ~ 30페이지
· 저자명 : 구자영, 박선자

초록

-Abstract- Background/Aims : Insulin and IGF-1 have trophic effects on a variety of tissue cells, and are known to increase protein and enzyme synthesis of pancreatic acinar cells. The purpose of this study is to assess the effects of insulin and IGF-1 on the growth of human pancreatic cancer cells (Mia PaCa-2 and CAPAN2).
Methods : Mia PaCa-2 ; 48h after platine cells (5-10x〖10〗^4 cells/well), medium was changed to serum-free medium. 48h after changing medium, insulin (0.1-10.0 ug/ml) or IGF-1 (0.1-100.0 ng/ml) was added. Cells were counted 4 days after treatment. In another experiment, IGF-1 receptor antibody (aIR-3) was added (5000 ng/ml) with or without insulin (1.0 ug.ml). CAPAN2 ; The experiment procedure was the same except cell number (10-20x〖10〗^4 cells/well) and day of cell counting (5 days). aIR-3 was added with or without insulin (1.0 ug/ml) or IGF1 (100.0 ng/ml). All experiments were repeated at least twice.
Results : 1) Insulin and IGF-1 stimulated the growth of both well-differentiated (CAPAN2) and undifferentiated (Mia PaCa-2) pancreatic cancer cell lines. 2) The concentrations at which insulin stimulated the growth of Mia PaCa-2 and CAPAN2 were 100.0 ng/ml and 1000ng/ml, respectively, which showed marked differences according to different cell lines. In contrast, IGF-1 stimulated the growth of Mia PaCa-2 and CAPAN2 at the same concentration, viz 10.0 ng/ml. 3) aIR-3 (5000 ng/ml) inhibited the growth-stimulatory effect of both insulin (1.0 ug/ml) and IGF-1 (100.0 ng/ml) on CAPAN2 cells, but no effects on the stimulation of growth by insulin (1.0 ug/ml) on Mia PaCa-2 cells.
Conclusions : Insulin and IGF-1 have growth-stimulatory effects on pancreatic cancer cells, and this effect may be exerted through different receptors according to different cancer cells. IGF-1 receptor antibodies may become a useful anticancer agent for pancreatic cancer.

영어초록

-Abstract- Background/Aims : Insulin and IGF-1 have trophic effects on a variety of tissue cells, and are known to increase protein and enzyme synthesis of pancreatic acinar cells. The purpose of this study is to assess the effects of insulin and IGF-1 on the growth of human pancreatic cancer cells (Mia PaCa-2 and CAPAN2).
Methods : Mia PaCa-2 ; 48h after platine cells (5-10x〖10〗^4 cells/well), medium was changed to serum-free medium. 48h after changing medium, insulin (0.1-10.0 ug/ml) or IGF-1 (0.1-100.0 ng/ml) was added. Cells were counted 4 days after treatment. In another experiment, IGF-1 receptor antibody (aIR-3) was added (5000 ng/ml) with or without insulin (1.0 ug.ml). CAPAN2 ; The experiment procedure was the same except cell number (10-20x〖10〗^4 cells/well) and day of cell counting (5 days). aIR-3 was added with or without insulin (1.0 ug/ml) or IGF1 (100.0 ng/ml). All experiments were repeated at least twice.
Results : 1) Insulin and IGF-1 stimulated the growth of both well-differentiated (CAPAN2) and undifferentiated (Mia PaCa-2) pancreatic cancer cell lines. 2) The concentrations at which insulin stimulated the growth of Mia PaCa-2 and CAPAN2 were 100.0 ng/ml and 1000ng/ml, respectively, which showed marked differences according to different cell lines. In contrast, IGF-1 stimulated the growth of Mia PaCa-2 and CAPAN2 at the same concentration, viz 10.0 ng/ml. 3) aIR-3 (5000 ng/ml) inhibited the growth-stimulatory effect of both insulin (1.0 ug/ml) and IGF-1 (100.0 ng/ml) on CAPAN2 cells, but no effects on the stimulation of growth by insulin (1.0 ug/ml) on Mia PaCa-2 cells.
Conclusions : Insulin and IGF-1 have growth-stimulatory effects on pancreatic cancer cells, and this effect may be exerted through different receptors according to different cancer cells. IGF-1 receptor antibodies may become a useful anticancer agent for pancreatic cancer.

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